Identification of essential genes in Staphylococcus aureus by construction and screening of conditional mutant library

Dezhong Yin, Yinduo Ji

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Antisense RNA technology has been used effectively to downregulate gene expression in a variety of bacterial systems. Regulated antisense RNA strategy provides an important approach to identify and characterize essential genes critical to bacterial growth in vitro and in vivo. This strategy allows selective genes to be turned on or off and to be expressed at certain levels. The availability of the Staphylococcus aureus (S. aureus) genome sequence makes it feasible to generate a gene-specific antisense RNA library. The combination of regulated antisense RNA technology and the gene-specific antisense RNA library allows for genome-wide analyses of functions of staphylococcal gene products for growth in culture and survival, during infection.

Original languageEnglish (US)
Title of host publicationMicrobial Gene Essentiality Protocols and Bioinformatics
Pages297-305
Number of pages9
StatePublished - Dec 1 2007

Publication series

NameMethods in Molecular Biology
Volume416
ISSN (Print)1064-3745

Keywords

  • Antisense RNA
  • Essential genes
  • Genome
  • Staphylococcus aureus

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