Identification of Differentially Expressed Transcripts and Pathways in Blood One Week and Six Months Following Implant of Left Ventricular Assist Devices

Adam Mitchell, Weihua Guan, Rodney Staggs, Aimee Hamel, Sameh Hozayen, Neeta Adhikari, Suzanne Grindle, Snider Desir, Ranjit John, Jennifer L. Hall, Peter Eckman

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Introduction:Continuous-flow left ventricular assist devices (LVADs) are an established therapy for patients with end-stage heart failure. The short- and long-term impact of these devices on peripheral blood gene expression has not been characterized, and may provide insight into the molecular pathways mediated in response to left ventricular remodeling and an improvement in overall systemic circulation. We performed RNA sequencing to identify genes and pathways influenced by these devices.Methods:RNA was extracted from blood of 9 heart failure patients (8 male) prior to LVAD implantation, and at 7 and 180 days postoperatively. Libraries were sequenced on an Illumina HiSeq2000 and sequences mapped to the human Ensembl GRCh37.67 genome assembly.Results:A specific set of genes involved in regulating cellular immune response, antigen presentation, and T cell activation and survival were down-regulated 7 days after LVAD placement. 6 months following LVAD placement, the expression levels of these genes were significantly increased; yet importantly, remained significantly lower than age and sex-matched samples from healthy controls.Conclusions:In summary, this genomic analysis identified a significant decrease in the expression of genes that promote a healthy immune response in patients with heart failure that was partially restored 6 months following LVAD implant.

Original languageEnglish (US)
Article numbere77951
JournalPloS one
Volume8
Issue number10
DOIs
StatePublished - Oct 21 2013

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