Identification of cytotoxic T lymphocyte epitopes of human herpesvirus 8

Fabiola Micheletti, Paolo Monini, Cinzia Fortini, Paola Rimessi, Martina Bazzaro, Massimo Andreoni, Massimo Giuliani, Serena Traniello, Barbara Ensoli, Riccardo Gavioli

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

The human herpesvirus 8 (HHV-8) is a human γ2-herpesvirus that is implicated in the development of Kaposi's sarcoma (KS), primary effusion lymphoma and Castelman's disease. Since the responses of cytotoxic T lymphocytes (CTL) play a key role in the control of herpesvirus infection, it is important to identify and to characterize the CTL target epitopes of HHV-8 viral antigens. In this study, using peptide-binding motifs, we selected potential human leucocyte antigen (HLA)-A2-binding peptides from kaposin A and glycoprotein H (gH), that are latent and lytic HHV-8 antigens, respectively. HLA-A2-binding peptides were tested for their capacity to induce CTL responses in HHV-8-negative healthy donors. By this approach, we found that the majority of individuals responded to two HHV-8-derived CTL epitopes, namely, VLLNGWRWRL (amino acids 16-25), which derives from kaposin A, and FLNWQNLLNV (amino acids 59-68), which derives from gH. In addition, memory CTL responses to these epitopes were detected in disease-free individuals infected by HHV-8 demonstrating that the two epitopes are relevant targets of CTL-mediated immunity in vivo. The identified epitopes may be investigated for the development of immunotherapeutic strategies against HHV-8-associated malignancies.

Original languageEnglish (US)
Pages (from-to)395-403
Number of pages9
JournalImmunology
Volume106
Issue number3
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint Dive into the research topics of 'Identification of cytotoxic T lymphocyte epitopes of human herpesvirus 8'. Together they form a unique fingerprint.

Cite this