Identification of catecholamine cell bodies in the pons and pons-mesencephalon junction of the cat brain, using tyrosine hydroxylase and dopamine-β-hydroxylase immunohistochemistry

S. Miachon; A. Berod; L. Leger; M. Chat; B. Hartman; J. F. Pujol

doi:10.1016/0006-8993(84)90445-1

Identification of catecholamine cell bodies in the pons and pons-mesencephalon junction of the cat brain, using tyrosine hydroxylase and dopamine-β-hydroxylase immunohistochemistry

S. Miachon
, A. Berod
, L. Leger
, M. Chat
, B. Hartman
, J. F. Pujol

Research output: Contribution to journal › Article › peer-review

46 Scopus citations

Abstract

Using a double labeling immunohistochemical method with antibodies to tyrosine hydroxylase and dopamine-β-hydroxylase, we reexamined the topographical distribution of dopaminergic (DA) and noradrenergic (NA) cells in the pons-mesencephalon junction of the cat. Besides DA cells of SN and its extensions, DA cells were also observed more caudally in nucleus raphe linearis intermedius and dorsalis, decussation of brachium conjunctivum, fasciculus longitudinalis medialis and periaqueductal gray. In nucleus locus coeruleus, cells exhibited variable levels of DBH, but dopaminergic cells were not evidenced in this structure.

Original language	English (US)
Pages (from-to)	369-374
Number of pages	6
Journal	Brain Research
Volume	305
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(84)90445-1
State	Published - Jul 9 1984
Externally published	Yes

Bibliographical note

Funding Information:
This work was supported by INSERM U 171, CNRS LA 162 and University Claude Bernard (U.E.R. Lyon-Nord). We wish to thank Dr M. J. Carew for revision of the English text.

Keywords

brainstem
catecholaminergic cell bodies
dopamine-β-hydroxylase
immunohistochemistry
tyrosine hydroxylase

Publisher link

10.1016/0006-8993(84)90445-1

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title = "Identification of catecholamine cell bodies in the pons and pons-mesencephalon junction of the cat brain, using tyrosine hydroxylase and dopamine-β-hydroxylase immunohistochemistry",

abstract = "Using a double labeling immunohistochemical method with antibodies to tyrosine hydroxylase and dopamine-β-hydroxylase, we reexamined the topographical distribution of dopaminergic (DA) and noradrenergic (NA) cells in the pons-mesencephalon junction of the cat. Besides DA cells of SN and its extensions, DA cells were also observed more caudally in nucleus raphe linearis intermedius and dorsalis, decussation of brachium conjunctivum, fasciculus longitudinalis medialis and periaqueductal gray. In nucleus locus coeruleus, cells exhibited variable levels of DBH, but dopaminergic cells were not evidenced in this structure.",

keywords = "brainstem, catecholaminergic cell bodies, dopamine-β-hydroxylase, immunohistochemistry, tyrosine hydroxylase",

author = "S. Miachon and A. Berod and L. Leger and M. Chat and B. Hartman and Pujol, \{J. F.\}",

note = "Funding Information: This work was supported by INSERM U 171, CNRS LA 162 and University Claude Bernard (U.E.R. Lyon-Nord). We wish to thank Dr M. J. Carew for revision of the English text.",

year = "1984",

month = jul,

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doi = "10.1016/0006-8993(84)90445-1",

language = "English (US)",

volume = "305",

pages = "369--374",

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TY - JOUR

T1 - Identification of catecholamine cell bodies in the pons and pons-mesencephalon junction of the cat brain, using tyrosine hydroxylase and dopamine-β-hydroxylase immunohistochemistry

AU - Miachon, S.

AU - Berod, A.

AU - Leger, L.

AU - Chat, M.

AU - Hartman, B.

AU - Pujol, J. F.

N1 - Funding Information: This work was supported by INSERM U 171, CNRS LA 162 and University Claude Bernard (U.E.R. Lyon-Nord). We wish to thank Dr M. J. Carew for revision of the English text.

PY - 1984/7/9

Y1 - 1984/7/9

N2 - Using a double labeling immunohistochemical method with antibodies to tyrosine hydroxylase and dopamine-β-hydroxylase, we reexamined the topographical distribution of dopaminergic (DA) and noradrenergic (NA) cells in the pons-mesencephalon junction of the cat. Besides DA cells of SN and its extensions, DA cells were also observed more caudally in nucleus raphe linearis intermedius and dorsalis, decussation of brachium conjunctivum, fasciculus longitudinalis medialis and periaqueductal gray. In nucleus locus coeruleus, cells exhibited variable levels of DBH, but dopaminergic cells were not evidenced in this structure.

AB - Using a double labeling immunohistochemical method with antibodies to tyrosine hydroxylase and dopamine-β-hydroxylase, we reexamined the topographical distribution of dopaminergic (DA) and noradrenergic (NA) cells in the pons-mesencephalon junction of the cat. Besides DA cells of SN and its extensions, DA cells were also observed more caudally in nucleus raphe linearis intermedius and dorsalis, decussation of brachium conjunctivum, fasciculus longitudinalis medialis and periaqueductal gray. In nucleus locus coeruleus, cells exhibited variable levels of DBH, but dopaminergic cells were not evidenced in this structure.

KW - brainstem

KW - catecholaminergic cell bodies

KW - dopamine-β-hydroxylase

KW - immunohistochemistry

KW - tyrosine hydroxylase

UR - https://www.scopus.com/pages/publications/0021252068

UR - https://www.scopus.com/pages/publications/0021252068#tab=citedBy

U2 - 10.1016/0006-8993(84)90445-1

DO - 10.1016/0006-8993(84)90445-1

M3 - Article

C2 - 6146389

AN - SCOPUS:0021252068

SN - 0006-8993

VL - 305

SP - 369

EP - 374

JO - Brain Research

JF - Brain Research

IS - 2

ER -

Identification of catecholamine cell bodies in the pons and pons-mesencephalon junction of the cat brain, using tyrosine hydroxylase and dopamine-β-hydroxylase immunohistochemistry

Abstract

Bibliographical note

Keywords

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