Identification of Candidate Susceptibility Genes to Puccinia graminis f. sp. tritici in Wheat

Eva C. Henningsen; Vahid Omidvar; Rafael Della Coletta; Jean Michel Michno; Erin Gilbert; Feng Li; Marisa E. Miller; Chad L. Myers; Sean P. Gordon; John P. Vogel; Brian J. Steffenson; Shahryar F. Kianian; Cory D. Hirsch; Melania Figueroa

doi:10.3389/fpls.2021.657796

Identification of Candidate Susceptibility Genes to Puccinia graminis f. sp. tritici in Wheat

Eva C. Henningsen, Vahid Omidvar, Rafael Della Coletta, Jean Michel Michno, Erin Gilbert, Feng Li, Marisa E. Miller, Chad L. Myers, Sean P. Gordon, John P. Vogel, Brian J. Steffenson, Shahryar F. Kianian, Cory D. Hirsch, Melania Figueroa

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3 Scopus citations

Abstract

Wheat stem rust disease caused by Puccinia graminis f. sp. tritici (Pgt) is a global threat to wheat production. Fast evolving populations of Pgt limit the efficacy of plant genetic resistance and constrain disease management strategies. Understanding molecular mechanisms that lead to rust infection and disease susceptibility could deliver novel strategies to deploy crop resistance through genetic loss of disease susceptibility. We used comparative transcriptome-based and orthology-guided approaches to characterize gene expression changes associated with Pgt infection in susceptible and resistant Triticum aestivum genotypes as well as the non-host Brachypodium distachyon. We targeted our analysis to genes with differential expression in T. aestivum and genes suppressed or not affected in B. distachyon and report several processes potentially linked to susceptibility to Pgt, such as cell death suppression and impairment of photosynthesis. We complemented our approach with a gene co-expression network analysis to identify wheat targets to deliver resistance to Pgt through removal or modification of putative susceptibility genes.

Original language	English (US)
Article number	657796
Journal	Frontiers in Plant Science
Volume	12
DOIs	https://doi.org/10.3389/fpls.2021.657796
State	Published - Apr 21 2021

Bibliographical note

Funding Information:
This work was supported by a seed grant from the Microbial Plant Genome Institute at The University of Minnesota, USDA-NIFA grant #2018-67013-27819, University of Minnesota Experimental Station USDA-NIFA Hatch project MIN-22-086, as well as the USDA-ARS/ The University of Minnesota Standard Cooperative Agreement (3002-11031-00053115) between SK and MF. EH is supported by a scholarship from the College of Food, Agriculture, and Natural Resource Science at the University of Minnesota. The work conducted by the United States DOE Joint Genome Institute is supported by the Office of Science of the United States Department of Energy under Contract no. DE-AC02-05CH11231.

Publisher Copyright:
© Copyright © 2021 Henningsen, Omidvar, Della Coletta, Michno, Gilbert, Li, Miller, Myers, Gordon, Vogel, Steffenson, Kianian, Hirsch and Figueroa.

Keywords

co-expression
non-host
rust (disease)
susceptibility
transcription
wheat

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10.3389/fpls.2021.657796

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Henningsen, E. C., Omidvar, V., Della Coletta, R., Michno, J. M., Gilbert, E., Li, F., Miller, M. E., Myers, C. L., Gordon, S. P., Vogel, J. P., Steffenson, B. J., Kianian, S. F., Hirsch, C. D., & Figueroa, M. (2021). Identification of Candidate Susceptibility Genes to Puccinia graminis f. sp. tritici in Wheat. Frontiers in Plant Science, 12, [657796]. https://doi.org/10.3389/fpls.2021.657796

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title = "Identification of Candidate Susceptibility Genes to Puccinia graminis f. sp. tritici in Wheat",

abstract = "Wheat stem rust disease caused by Puccinia graminis f. sp. tritici (Pgt) is a global threat to wheat production. Fast evolving populations of Pgt limit the efficacy of plant genetic resistance and constrain disease management strategies. Understanding molecular mechanisms that lead to rust infection and disease susceptibility could deliver novel strategies to deploy crop resistance through genetic loss of disease susceptibility. We used comparative transcriptome-based and orthology-guided approaches to characterize gene expression changes associated with Pgt infection in susceptible and resistant Triticum aestivum genotypes as well as the non-host Brachypodium distachyon. We targeted our analysis to genes with differential expression in T. aestivum and genes suppressed or not affected in B. distachyon and report several processes potentially linked to susceptibility to Pgt, such as cell death suppression and impairment of photosynthesis. We complemented our approach with a gene co-expression network analysis to identify wheat targets to deliver resistance to Pgt through removal or modification of putative susceptibility genes.",

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author = "Henningsen, {Eva C.} and Vahid Omidvar and {Della Coletta}, Rafael and Michno, {Jean Michel} and Erin Gilbert and Feng Li and Miller, {Marisa E.} and Myers, {Chad L.} and Gordon, {Sean P.} and Vogel, {John P.} and Steffenson, {Brian J.} and Kianian, {Shahryar F.} and Hirsch, {Cory D.} and Melania Figueroa",

note = "Funding Information: This work was supported by a seed grant from the Microbial Plant Genome Institute at The University of Minnesota, USDA-NIFA grant #2018-67013-27819, University of Minnesota Experimental Station USDA-NIFA Hatch project MIN-22-086, as well as the USDA-ARS/ The University of Minnesota Standard Cooperative Agreement (3002-11031-00053115) between SK and MF. EH is supported by a scholarship from the College of Food, Agriculture, and Natural Resource Science at the University of Minnesota. The work conducted by the United States DOE Joint Genome Institute is supported by the Office of Science of the United States Department of Energy under Contract no. DE-AC02-05CH11231. Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Henningsen, Omidvar, Della Coletta, Michno, Gilbert, Li, Miller, Myers, Gordon, Vogel, Steffenson, Kianian, Hirsch and Figueroa.",

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T1 - Identification of Candidate Susceptibility Genes to Puccinia graminis f. sp. tritici in Wheat

AU - Henningsen, Eva C.

AU - Omidvar, Vahid

AU - Della Coletta, Rafael

AU - Michno, Jean Michel

AU - Gilbert, Erin

AU - Li, Feng

AU - Miller, Marisa E.

AU - Myers, Chad L.

AU - Gordon, Sean P.

AU - Vogel, John P.

AU - Steffenson, Brian J.

AU - Kianian, Shahryar F.

AU - Hirsch, Cory D.

AU - Figueroa, Melania

N1 - Funding Information: This work was supported by a seed grant from the Microbial Plant Genome Institute at The University of Minnesota, USDA-NIFA grant #2018-67013-27819, University of Minnesota Experimental Station USDA-NIFA Hatch project MIN-22-086, as well as the USDA-ARS/ The University of Minnesota Standard Cooperative Agreement (3002-11031-00053115) between SK and MF. EH is supported by a scholarship from the College of Food, Agriculture, and Natural Resource Science at the University of Minnesota. The work conducted by the United States DOE Joint Genome Institute is supported by the Office of Science of the United States Department of Energy under Contract no. DE-AC02-05CH11231. Publisher Copyright: © Copyright © 2021 Henningsen, Omidvar, Della Coletta, Michno, Gilbert, Li, Miller, Myers, Gordon, Vogel, Steffenson, Kianian, Hirsch and Figueroa.

PY - 2021/4/21

Y1 - 2021/4/21

N2 - Wheat stem rust disease caused by Puccinia graminis f. sp. tritici (Pgt) is a global threat to wheat production. Fast evolving populations of Pgt limit the efficacy of plant genetic resistance and constrain disease management strategies. Understanding molecular mechanisms that lead to rust infection and disease susceptibility could deliver novel strategies to deploy crop resistance through genetic loss of disease susceptibility. We used comparative transcriptome-based and orthology-guided approaches to characterize gene expression changes associated with Pgt infection in susceptible and resistant Triticum aestivum genotypes as well as the non-host Brachypodium distachyon. We targeted our analysis to genes with differential expression in T. aestivum and genes suppressed or not affected in B. distachyon and report several processes potentially linked to susceptibility to Pgt, such as cell death suppression and impairment of photosynthesis. We complemented our approach with a gene co-expression network analysis to identify wheat targets to deliver resistance to Pgt through removal or modification of putative susceptibility genes.

AB - Wheat stem rust disease caused by Puccinia graminis f. sp. tritici (Pgt) is a global threat to wheat production. Fast evolving populations of Pgt limit the efficacy of plant genetic resistance and constrain disease management strategies. Understanding molecular mechanisms that lead to rust infection and disease susceptibility could deliver novel strategies to deploy crop resistance through genetic loss of disease susceptibility. We used comparative transcriptome-based and orthology-guided approaches to characterize gene expression changes associated with Pgt infection in susceptible and resistant Triticum aestivum genotypes as well as the non-host Brachypodium distachyon. We targeted our analysis to genes with differential expression in T. aestivum and genes suppressed or not affected in B. distachyon and report several processes potentially linked to susceptibility to Pgt, such as cell death suppression and impairment of photosynthesis. We complemented our approach with a gene co-expression network analysis to identify wheat targets to deliver resistance to Pgt through removal or modification of putative susceptibility genes.

KW - co-expression

KW - non-host

KW - rust (disease)

KW - susceptibility

KW - transcription

KW - wheat

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DO - 10.3389/fpls.2021.657796

M3 - Article

C2 - 33968112

AN - SCOPUS:85105378433

SN - 1664-462X

VL - 12

JO - Frontiers in Plant Science

JF - Frontiers in Plant Science

M1 - 657796

ER -

Identification of Candidate Susceptibility Genes to Puccinia graminis f. sp. tritici in Wheat

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