Identification of bacteria in formalin-fixed, paraffin-embedded heart valve tissue via 16S rRNA gene nucleotide sequencing

Kavita Imrit; Michael Goldfischer; Jie Wang; Jaime Green; Jerome Levine; Joseph Lombardo; Tao Hong

doi:10.1128/JCM.00572-06

Identification of bacteria in formalin-fixed, paraffin-embedded heart valve tissue via 16S rRNA gene nucleotide sequencing

Kavita Imrit
, Michael Goldfischer
, Jie Wang
, Jaime Green
, Jerome Levine
, Joseph Lombardo
, Tao Hong

Medicine - Infectious Disease and International Medicine

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

We applied 16S rRNA gene sequencing to identify bacterial species present in formalin-fixed, paraffin-embedded heart valve tissue. In 40% (12/30) of the cases, we were able to identify the bacterium to the species-genus level. For more recent cases (≤4 years), the success rate was significantly improved, to 70% (P < 0.001).

Original language	English (US)
Pages (from-to)	2609-2611
Number of pages	3
Journal	Journal of clinical microbiology
Volume	44
Issue number	7
DOIs	https://doi.org/10.1128/JCM.00572-06
State	Published - Jul 2006

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10.1128/JCM.00572-06

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title = "Identification of bacteria in formalin-fixed, paraffin-embedded heart valve tissue via 16S rRNA gene nucleotide sequencing",

abstract = "We applied 16S rRNA gene sequencing to identify bacterial species present in formalin-fixed, paraffin-embedded heart valve tissue. In 40\% (12/30) of the cases, we were able to identify the bacterium to the species-genus level. For more recent cases (≤4 years), the success rate was significantly improved, to 70\% (P < 0.001).",

author = "Kavita Imrit and Michael Goldfischer and Jie Wang and Jaime Green and Jerome Levine and Joseph Lombardo and Tao Hong",

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AU - Imrit, Kavita

AU - Goldfischer, Michael

AU - Wang, Jie

AU - Green, Jaime

AU - Levine, Jerome

AU - Lombardo, Joseph

AU - Hong, Tao

PY - 2006/7

Y1 - 2006/7

N2 - We applied 16S rRNA gene sequencing to identify bacterial species present in formalin-fixed, paraffin-embedded heart valve tissue. In 40% (12/30) of the cases, we were able to identify the bacterium to the species-genus level. For more recent cases (≤4 years), the success rate was significantly improved, to 70% (P < 0.001).

AB - We applied 16S rRNA gene sequencing to identify bacterial species present in formalin-fixed, paraffin-embedded heart valve tissue. In 40% (12/30) of the cases, we were able to identify the bacterium to the species-genus level. For more recent cases (≤4 years), the success rate was significantly improved, to 70% (P < 0.001).

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UR - https://www.scopus.com/pages/publications/33746216114#tab=citedBy

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DO - 10.1128/JCM.00572-06

M3 - Article

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AN - SCOPUS:33746216114

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ER -

Identification of bacteria in formalin-fixed, paraffin-embedded heart valve tissue via 16S rRNA gene nucleotide sequencing

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