Abstract
Immunoblot analysis of antiserum to rat central nervous system (CNS) myelin revealed antibodies to myelin basic protein (MBP), proteolipid protein (PLP), and numerous high molecular weight proteins. In addition, anti-CNS myelin serum exclusively immunostained 4 basic proteins of rat peripheral neuvous system (PNS) myelin. Similarly, anti-PNS myelin sera immunostained many high molecular weight proteins in both CNS and PNS myelin in addition to P0 and 4 basic proteins. Purified MBP and PLP were immunostained by anti-CNS myelin sera and MBP and P0 by anti-PNS myelin sera, indicating that antigenic sites are preserved during protein purification. Immunohistochemical localization with antisera was confined to the myelin sheath except that antisera to CNS myelin also stained oligodendrocytes during the active period of myelination. While anti-CNS myelin sera specifically demyelinated centrally myelinated fibers in culture, none of the anti-PNS myelin sera used here demyelinated organotypic spinal cord-dorsal root ganglion cultures.
Original language | English (US) |
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Pages (from-to) | 191-200 |
Number of pages | 10 |
Journal | Brain Research |
Volume | 307 |
Issue number | 1-2 |
DOIs | |
State | Published - Jul 30 1984 |
Externally published | Yes |
Keywords
- central nervous system
- demyelinating antibodies
- immunoblot technique
- immunohistochemistry
- myelin proteins
- nerve tissue cultures
- peripheral nervous system