Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana

William M. Gray; J. Carlos Del Pozo; Loni Walker; Lawrence Hobbie; Eddy Risseeuw; Travis Banks; William L. Crosby; Ming Yang; Hong Ma; Mark Estelle

doi:10.1101/gad.13.13.1678

Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana

William M. Gray, J. Carlos Del Pozo, Loni Walker, Lawrence Hobbie, Eddy Risseeuw, Travis Banks, William L. Crosby, Ming Yang, Hong Ma, Mark Estelle

Plant and Microbial Biology

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Abstract

The plant hormone auxin regulates diverse aspects of plant growth and development. We report that in Arabidopsis, auxin response is dependent on a ubiquitin-ligase (E3) complex called SCF(TIR1). The complex consists of proteins related to yeast Skp1p and Cdc53p called ASK and AtCUL1, respectively, as well as the F-box protein TIR1. Mutations in either ASK1 or TIR1 result in decreased auxin response. Further, overexpression of TIR1 promotes auxin response suggesting that SCF(TIR1) is limiting for the response. These results provide new support for a model in which auxin action depends on the regulated proteolysis of repressor proteins.

Original language	English (US)
Pages (from-to)	1678-1691
Number of pages	14
Journal	Genes and Development
Volume	13
Issue number	13
DOIs	https://doi.org/10.1101/gad.13.13.1678
State	Published - Jul 1 1999

Keywords

Arabidopsis
Auxin
Proteolysis
SCF
TIR1
Ubiquitin-ligase

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10.1101/gad.13.13.1678

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abstract = "The plant hormone auxin regulates diverse aspects of plant growth and development. We report that in Arabidopsis, auxin response is dependent on a ubiquitin-ligase (E3) complex called SCF(TIR1). The complex consists of proteins related to yeast Skp1p and Cdc53p called ASK and AtCUL1, respectively, as well as the F-box protein TIR1. Mutations in either ASK1 or TIR1 result in decreased auxin response. Further, overexpression of TIR1 promotes auxin response suggesting that SCF(TIR1) is limiting for the response. These results provide new support for a model in which auxin action depends on the regulated proteolysis of repressor proteins.",

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AU - Gray, William M.

AU - Del Pozo, J. Carlos

AU - Walker, Loni

AU - Hobbie, Lawrence

AU - Risseeuw, Eddy

AU - Banks, Travis

AU - Crosby, William L.

AU - Yang, Ming

AU - Ma, Hong

AU - Estelle, Mark

PY - 1999/7/1

Y1 - 1999/7/1

N2 - The plant hormone auxin regulates diverse aspects of plant growth and development. We report that in Arabidopsis, auxin response is dependent on a ubiquitin-ligase (E3) complex called SCF(TIR1). The complex consists of proteins related to yeast Skp1p and Cdc53p called ASK and AtCUL1, respectively, as well as the F-box protein TIR1. Mutations in either ASK1 or TIR1 result in decreased auxin response. Further, overexpression of TIR1 promotes auxin response suggesting that SCF(TIR1) is limiting for the response. These results provide new support for a model in which auxin action depends on the regulated proteolysis of repressor proteins.

AB - The plant hormone auxin regulates diverse aspects of plant growth and development. We report that in Arabidopsis, auxin response is dependent on a ubiquitin-ligase (E3) complex called SCF(TIR1). The complex consists of proteins related to yeast Skp1p and Cdc53p called ASK and AtCUL1, respectively, as well as the F-box protein TIR1. Mutations in either ASK1 or TIR1 result in decreased auxin response. Further, overexpression of TIR1 promotes auxin response suggesting that SCF(TIR1) is limiting for the response. These results provide new support for a model in which auxin action depends on the regulated proteolysis of repressor proteins.

KW - Arabidopsis

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KW - Proteolysis

KW - SCF

KW - TIR1

KW - Ubiquitin-ligase

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Identification of an SCF ubiquitin-ligase complex required for auxin response in Arabidopsis thaliana

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