Identification of a Zds-like gene ZDS3 as a new mediator of stress resistance, capsule formation and virulence of the human pathogenic yeast Cryptococcus neoformans

Zhongming Li, Zhixiong Sun, Dong Li, Jiao Pan, Xudong Zhu

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The fungal Zds proteins are regulators of the serine/threonine phosphatase 2A (PP2A) and the protein kinase A. Here, we characterize a Zds-like gene ZDS3 that plays a broad range of roles in the basidiomycetous pathogenic yeast Cryptococcus neoformans. ZDS3 harbors the conserved activation domain ZDS-C of Zds proteins. By gene disruption, ZDS3 is shown to play roles in capsule production, cell wall integrity, growth at a high temperature, resistance to H 2O 2 stress, osmotic pressures and glucose-dependent invasive growth on the agar. As a consequence, the disruption of ZDS3 resulted in complete loss of virulence in a mouse cryptococcosis model. The data suggest that ZDS3 is a novel mediator of the virulence of C. neoformans. Zds3 may serve as an antifungal drug target as no homologs are found in mammals. FEMS Yeast Research

Original languageEnglish (US)
Pages (from-to)529-539
Number of pages11
JournalFEMS yeast research
Volume11
Issue number7
DOIs
StatePublished - Nov 2011

Keywords

  • Capsule
  • Cryptococcus neoformans
  • Virulence factor
  • Zds

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