Control of kappa immunoglobulin light-chain gene expression requires the interaction of tissue specific and developmentally regulated DNA-binding proteins with the kappa gene enhancer. Deletion of enhancer sequences upstream from the NF-kB binding site has been shown to impair enhancer function, implying additional proteins may interact with these sequences. in surveying this region for sites of protein binding, a novel DNA-protein interaction, designated kBF-A, was detected. The binding activity of this factor appears to be specific to activated pre-B cells and correlates with high level induction of kappa transcription in these cells.
Bibliographical noteFunding Information:
We thank L. King for excellent technical assistance and are grateful to K. Conklin and H. Towle for critical review of the manuscript. This work is supported by a pre-doctoral training grant to K.N. and by grant GM37687 to B.V.N. from the National Institutes of Health.