Identification of a second inducible DNA-protein interaction in the kappa immunoglobulin enhancer

Keats Neims, Robert Hromas, Brian van Ness

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Control of kappa immunoglobulin light-chain gene expression requires the interaction of tissue specific and developmentally regulated DNA-binding proteins with the kappa gene enhancer. Deletion of enhancer sequences upstream from the NF-kB binding site has been shown to impair enhancer function, implying additional proteins may interact with these sequences. in surveying this region for sites of protein binding, a novel DNA-protein interaction, designated kBF-A, was detected. The binding activity of this factor appears to be specific to activated pre-B cells and correlates with high level induction of kappa transcription in these cells.

Original languageEnglish (US)
Pages (from-to)1037-1043
Number of pages7
JournalNucleic acids research
Volume18
Issue number4
DOIs
StatePublished - Feb 25 1990

Bibliographical note

Funding Information:
We thank L. King for excellent technical assistance and are grateful to K. Conklin and H. Towle for critical review of the manuscript. This work is supported by a pre-doctoral training grant to K.N. and by grant GM37687 to B.V.N. from the National Institutes of Health.

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