Identification of a residue affecting fatty alcohol selectivity in wax ester synthase

Brett M. Barney, Rachel L. Mann, Janet M. Ohlert

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

The terminal enzyme in the bacterial wax ester biosynthetic pathway is the bifunctional wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase (WS/DGAT), which utilizes a fatty alcohol and a fatty acyl-coenzyme A (CoA) to synthesize the corresponding wax ester. In this report, we identify a specific residue in WS/DGAT enzymes obtained from Marinobacter aquaeolei VT8 and Acinetobacter baylyi that alters fatty alcohol selectivity and kinetic parameters when modified to alternative residues.

Original languageEnglish (US)
Pages (from-to)396-399
Number of pages4
JournalApplied and environmental microbiology
Volume79
Issue number1
DOIs
StatePublished - Jan 2013

Fingerprint Dive into the research topics of 'Identification of a residue affecting fatty alcohol selectivity in wax ester synthase'. Together they form a unique fingerprint.

Cite this