Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis

Srinand Sreevatsan, Patricio Escalante, X. Pan, Duncan A. Gillies, Subeeh Siddiqui, Christina N. Khalaf, Barry N. Kreiswirth, Pablo Bifani, L. Garry Adams, Thomas Ficht, Veera S. Perumaalla, M. Donald Cave, Jan D.A. Van Embden, James M. Musser

Research output: Contribution to journalArticlepeer-review

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Automated sequence analysis of a 410-bp region of the oxyR gene in 105 Mycobacterium tuberculosis complex isolates identified a polymorphic nucleotide that differentiated Mycobacterium bovis isolates from other complex members. All 29 M. bovis isolates sequenced had an adenine residue at nucleotide 285, whereas all 76 other complex isolates had a guanine residue. PCR-restriction fragment length polymorphism analysis of oxyR with restriction endonuclease AluI in an additional 255 complex isolates from widespread intercontinental sources confirmed and extended the unique association of adenine at position 285 with M. bovis isolates.

Original languageEnglish (US)
Pages (from-to)2007-2010
Number of pages4
JournalJournal of clinical microbiology
Issue number8
StatePublished - Aug 1996


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