Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis

Srinand Sreevatsan; Patricio Escalante; X. Pan; Duncan A. Gillies; Subeeh Siddiqui; Christina N. Khalaf; Barry N. Kreiswirth; Pablo Bifani; L. Garry Adams; Thomas Ficht; Veera S. Perumaalla; M. Donald Cave; Jan D.A. Van Embden; James M. Musser

doi:10.1128/jcm.34.8.2007-2010.1996

Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis

Srinand Sreevatsan, Patricio Escalante, X. Pan, Duncan A. Gillies, Subeeh Siddiqui, Christina N. Khalaf, Barry N. Kreiswirth, Pablo Bifani, L. Garry Adams, Thomas Ficht, Veera S. Perumaalla, M. Donald Cave, Jan D.A. Van Embden, James M. Musser

Veterinary Population Medicine

Research output: Contribution to journal › Article › peer-review

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Abstract

Automated sequence analysis of a 410-bp region of the oxyR gene in 105 Mycobacterium tuberculosis complex isolates identified a polymorphic nucleotide that differentiated Mycobacterium bovis isolates from other complex members. All 29 M. bovis isolates sequenced had an adenine residue at nucleotide 285, whereas all 76 other complex isolates had a guanine residue. PCR-restriction fragment length polymorphism analysis of oxyR with restriction endonuclease AluI in an additional 255 complex isolates from widespread intercontinental sources confirmed and extended the unique association of adenine at position 285 with M. bovis isolates.

Original language	English (US)
Pages (from-to)	2007-2010
Number of pages	4
Journal	Journal of clinical microbiology
Volume	34
Issue number	8
DOIs	https://doi.org/10.1128/jcm.34.8.2007-2010.1996
State	Published - Aug 1996

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Sreevatsan, S., Escalante, P., Pan, X., Gillies, D. A., Siddiqui, S., Khalaf, C. N., Kreiswirth, B. N., Bifani, P., Adams, L. G., Ficht, T., Perumaalla, V. S., Cave, M. D., Van Embden, J. D. A., & Musser, J. M. (1996). Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis. Journal of clinical microbiology, 34(8), 2007-2010. https://doi.org/10.1128/jcm.34.8.2007-2010.1996

Sreevatsan, S, Escalante, P, Pan, X, Gillies, DA, Siddiqui, S, Khalaf, CN, Kreiswirth, BN, Bifani, P, Adams, LG, Ficht, T, Perumaalla, VS, Cave, MD, Van Embden, JDA & Musser, JM 1996, 'Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis', Journal of clinical microbiology, vol. 34, no. 8, pp. 2007-2010. https://doi.org/10.1128/jcm.34.8.2007-2010.1996

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abstract = "Automated sequence analysis of a 410-bp region of the oxyR gene in 105 Mycobacterium tuberculosis complex isolates identified a polymorphic nucleotide that differentiated Mycobacterium bovis isolates from other complex members. All 29 M. bovis isolates sequenced had an adenine residue at nucleotide 285, whereas all 76 other complex isolates had a guanine residue. PCR-restriction fragment length polymorphism analysis of oxyR with restriction endonuclease AluI in an additional 255 complex isolates from widespread intercontinental sources confirmed and extended the unique association of adenine at position 285 with M. bovis isolates.",

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AU - Siddiqui, Subeeh

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AU - Kreiswirth, Barry N.

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AU - Adams, L. Garry

AU - Ficht, Thomas

AU - Perumaalla, Veera S.

AU - Cave, M. Donald

AU - Van Embden, Jan D.A.

AU - Musser, James M.

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AB - Automated sequence analysis of a 410-bp region of the oxyR gene in 105 Mycobacterium tuberculosis complex isolates identified a polymorphic nucleotide that differentiated Mycobacterium bovis isolates from other complex members. All 29 M. bovis isolates sequenced had an adenine residue at nucleotide 285, whereas all 76 other complex isolates had a guanine residue. PCR-restriction fragment length polymorphism analysis of oxyR with restriction endonuclease AluI in an additional 255 complex isolates from widespread intercontinental sources confirmed and extended the unique association of adenine at position 285 with M. bovis isolates.

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Identification of a polymorphic nucleotide in oxyR specific for Mycobacterium bovis

Abstract

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