Identification of a human RAD52 pseudogene located on chromosome 2

Brad L. Johnson, Colin R Campbell

Research output: Contribution to journalArticlepeer-review


A human testis cDNA library was screened with a hybridization probe encoding the mouse RAD52 gene. Two classes of clones were identified, one derived from the human RAD52 homolog (hRAD52), the other derived from a pseudogene. In addition to many point mutations, several of which encode stop codons, the pseudogene contains a number of frame shifts and a 103-bp deletion. We further determined that the pseudogene is processed and is located on human chromosome 2, in contrast to hRAD52 which is found on chromosome 12. Reverse transcription-PCR analysis of cultured human diploid fibroblasts, as well as fibrosarcoma cells, revealed that while hRAD52 is expressed at low, but detectable levels in these cells, the pseudogene is not.

Original languageEnglish (US)
Pages (from-to)229-232
Number of pages4
Issue number2
StatePublished - Mar 9 1996


  • DNA repair
  • Homologous recombination
  • Molecular genetics


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