Identification of a diagnostic marker to detect freshwater cyanophages of filamentous cyanobacteria

Andrea C. Baker, Victoria J. Goddard, Joanne Davy, Declan C. Schroeder, David G. Adams, William H. Wilson

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Cyanophages are viruses that infect the cyanobacteria, globally important photosynthetic microorganisms. Cyanophages are considered significant components of microbial communities, playing major roles in influencing host community diversity and primary productivity, terminating cyanobacterial water blooms, and influencing biogeochemical cycles. Cyanophages are ubiquitous in both marine and freshwater systems; however, the majority of molecular research has been biased toward the study of marine cyanophages. In this study, a diagnostic probe was developed to detect freshwater cyanophages in natural waters. Oligonucleotide PCR-based primers were designed to specifically amplify the major capsid protein gene from previously characterized freshwater cyanomyoviruses that are infectious to the filamentous, nitrogen-fixing cyanobacterial genera Anabaena and Nostoc. The primers were also successful in yielding PCR products from mixed virus communities concentrated from water samples collected from freshwater lakes in the United Kingdom. The probes are thought to provide a useful tool for the investigation of cyanophage diversity in freshwater environments.

Original languageEnglish (US)
Pages (from-to)5713-5719
Number of pages7
JournalApplied and environmental microbiology
Volume72
Issue number9
DOIs
StatePublished - Sep 2006

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