Identification of a cell-of-origin for fibroblasts comprising the fibrotic reticulum in idiopathic pulmonary fibrosis

Hong Xia, Vidya Bodempudi, Alexey Benyumov, Polla Hergert, Damien Tank, Jeremy Herrera, Jeff Braziunas, Ola Larsson, Matthew Parker, Daniel Rossi, Karen Smith, Mark Peterson, Andrew Limper, Jose Jessurun, John E Connett, David H Ingbar, Sem Phan, Peter B Bitterman, Craig A Henke

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Idiopathic pulmonary fibrosis (IPF) is a progressive disease of the middle aged and elderly with a prevalence of one million persons worldwide. The fibrosis spreads from affected alveoli into contiguous alveoli, creating a reticular network that leads to death by asphyxiation. Lung fibroblasts from patients with IPF have phenotypic hallmarks, distinguishing them from their normal counterparts: pathologically activated Akt signaling axis, increased collagen and α-smooth muscle actin expression, distinct gene expression profile, and ability to form fibrotic lesions in model organisms. Despite the centrality of these fibroblasts in disease pathogenesis, their origin remains uncertain. Here, we report the identification of cells in the lungs of patients with IPF with the properties of mesenchymal progenitors. In contrast to progenitors isolated from nonfibrotic lungs, IPF mesenchymal progenitor cells produce daughter cells manifesting the full spectrum of IPF hallmarks, including the ability to form fibrotic lesions in zebrafish embryos and mouse lungs, and a transcriptional profile reflecting these properties. Morphological analysis of IPF lung tissue revealed that mesenchymal progenitor cells and cells with the characteristics of their progeny comprised the fibrotic reticulum. These data establish that the lungs of patients with IPF contain pathological mesenchymal progenitor cells that are cells of origin for fibrosis-mediating fibroblasts. These fibrogenic mesenchymal progenitors and their progeny represent an unexplored target for novel therapies to interdict fibrosis.

Original languageEnglish (US)
Pages (from-to)1369-1383
Number of pages15
JournalAmerican Journal of Pathology
Volume184
Issue number5
DOIs
StatePublished - Jan 1 2014

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Reticulum
Idiopathic Pulmonary Fibrosis
Fibroblasts
Lung
Mesenchymal Stromal Cells
Fibrosis
Asphyxia
Zebrafish
Transcriptome
Smooth Muscle
Actins
Collagen
Embryonic Structures

Cite this

Identification of a cell-of-origin for fibroblasts comprising the fibrotic reticulum in idiopathic pulmonary fibrosis. / Xia, Hong; Bodempudi, Vidya; Benyumov, Alexey; Hergert, Polla; Tank, Damien; Herrera, Jeremy; Braziunas, Jeff; Larsson, Ola; Parker, Matthew; Rossi, Daniel; Smith, Karen; Peterson, Mark; Limper, Andrew; Jessurun, Jose; Connett, John E; Ingbar, David H; Phan, Sem; Bitterman, Peter B; Henke, Craig A.

In: American Journal of Pathology, Vol. 184, No. 5, 01.01.2014, p. 1369-1383.

Research output: Contribution to journalArticle

Xia, H, Bodempudi, V, Benyumov, A, Hergert, P, Tank, D, Herrera, J, Braziunas, J, Larsson, O, Parker, M, Rossi, D, Smith, K, Peterson, M, Limper, A, Jessurun, J, Connett, JE, Ingbar, DH, Phan, S, Bitterman, PB & Henke, CA 2014, 'Identification of a cell-of-origin for fibroblasts comprising the fibrotic reticulum in idiopathic pulmonary fibrosis', American Journal of Pathology, vol. 184, no. 5, pp. 1369-1383. https://doi.org/10.1016/j.ajpath.2014.01.012
Xia, Hong ; Bodempudi, Vidya ; Benyumov, Alexey ; Hergert, Polla ; Tank, Damien ; Herrera, Jeremy ; Braziunas, Jeff ; Larsson, Ola ; Parker, Matthew ; Rossi, Daniel ; Smith, Karen ; Peterson, Mark ; Limper, Andrew ; Jessurun, Jose ; Connett, John E ; Ingbar, David H ; Phan, Sem ; Bitterman, Peter B ; Henke, Craig A. / Identification of a cell-of-origin for fibroblasts comprising the fibrotic reticulum in idiopathic pulmonary fibrosis. In: American Journal of Pathology. 2014 ; Vol. 184, No. 5. pp. 1369-1383.
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