Identification, expression analysis and functional verification of two genes encoding small heat shock proteins in the western flower thrips, Frankliniella occidentalis (Pergande)

Jia Wen Yuan, Hai Xia Song, Ya Wen Chang, Fei Yang, Hong Fang Xie, Wei Rong Gong, Yu Zhou Du

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Small heat shock proteins (sHSPs) help prevent the irreversible aggregation of denatured proteins that occurs in response to organismal stress. In this study, we identified two intron-free genes encoding sHSPs from Frankliniella occidentalis; these were designated FoHSP11.6 and FoHSP28.0 and belonged to an atypical and typical sHSP family, respectively. Both FoHSPs were transcribed in all developmental stages of F. occidentalis with the highest expression levels in pupae and adults and greater expression in males than females. Although the FoHSPs had different temperature-induced expression profiles, they were generally induced by both low and high temperatures and reached maximal expression levels after 0.5–1 h of temperature stress. The FoHSPs expression levels in pupae were induced by drought and high humidity, and higher expression levels were correlated with lower survival rates. The thermotolerance of F. occidentalis decreased when theFoHSPs were silenced by RNA interference. Our results show that FoHSP11.6 and FoHSP28.0 are involved in the response to temperature and drought and may also function in growth and development of F. occidentalis.

Original languageEnglish (US)
Pages (from-to)74-84
Number of pages11
JournalInternational Journal of Biological Macromolecules
Volume211
DOIs
StatePublished - Jun 30 2022
Externally publishedYes

Bibliographical note

Funding Information:
This research was funded by Special Fund for Jiangsu Agricultural Industry Technology System ( JATS [2021] 346 ), Special Fund for Detection and Identification of Sudden Major Agricultural Pests in Nanjing Area and Special Fund for Agro-Scientific Research in the Public Interest of China ( 201103026 , 200803025 ). We also express our deep gratitude to the Testing Center of Yangzhou University.

Funding Information:
This research was funded by Special Fund for Jiangsu Agricultural Industry Technology System (JATS [2021] 346), Special Fund for Detection and Identification of Sudden Major Agricultural Pests in Nanjing Area and Special Fund for Agro-Scientific Research in the Public Interest of China (201103026, 200803025). We also express our deep gratitude to the Testing Center of Yangzhou University.

Publisher Copyright:
© 2022 Elsevier B.V.

Keywords

  • Frankliniella occidentalis
  • HSP10/CPN10
  • Small heat shock protein (sHSP)

PubMed: MeSH publication types

  • Journal Article

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