Identification, characterization and putative function of HvRin4, a barley homolog of Arabidopsis Rin4

Upinder Gill, Jayaveeramuthu Nirmala, Robert Brueggeman, Andris Kleinhofs

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


RIN4 is a signaling molecule that plays a key role in disease resistance responses in plants. Barley RIN4 (HvRIN4) was first identified as an interactor of the stem rust resistance protein RPG1 in a yeast two-hybrid system. Therefore, we isolated, characterized and studied HvRIN4 role in barley. Yeast two hybrid analysis, employing several deletion mutants of HvRIN4 revealed that the C-terminal end and a partial deletion of N-terminal end of HvRIN4 are important for interacting with RPG1. HvRIN4 interactions with RPG1 mutated proteins, which inactivate autophosphorylation, suggested that autophosphorylation or the amino acids required for autophosphorylation of RPG1 are essential for interaction.

Original languageEnglish (US)
Pages (from-to)41-49
Number of pages9
JournalPhysiological and Molecular Plant Pathology
StatePublished - Oct 2012

Bibliographical note

Funding Information:
Research was supported by National Research Initiative of the United States Department of Agriculture Co-operative State Research, Education, and Extension Service Grant No. 2007-35301-18205 to A.K and B.J.S.


  • Disease resistance
  • HvRin4
  • Protein-protein interactions
  • RPG1
  • Stem rust
  • Virus induced gene silencing (VIGS)


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