Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC)

Hongjun Shu; Yingxin Zhao; Ge Zhou; She Chen; Julia D. Wulfkuhle; Dennis C. Sgroi; Henry Kruztsch; Kelly McLean; Melodie Knowlton; Aysegul Sahin; Yue Chen; Emanuel Petricoin; Patricia S. Steeg; Yingming Zhao

Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC)

Hongjun Shu
, Yingxin Zhao
, Ge Zhou
, She Chen
, Julia D. Wulfkuhle
, Dennis C. Sgroi
, Henry Kruztsch
, Kelly McLean
, Melodie Knowlton
, Aysegul Sahin
, Yue Chen
, Emanuel Petricoin
, Patricia S. Steeg
, Yingming Zhao

Biochemistry, Molecular Biology, and Biophysics (CBS)

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

A proteomic comparison of matched normal and cancer cells was presented, and the new facts of the transition of breast and esophageal cancer were revealed. Pure populations of normal and cancer cells were obtained using laser capture microdissection (LCM). Protein sample preparation, 2D-gel electrophoresis, and mass spectrometry of protein identification, and immunohistochemistry were performed. It was found that the 3D simulation of up and down-regulated protein spots. Results show the spot pairs of up-regulated fascin, equally expressed tubulin and down regulated annexin.

Original language	English (US)
Title of host publication	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Pages	227-228
Number of pages	2
State	Published - Dec 1 2002
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: Jun 2 2002 → Jun 6 2002

Other

Other	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	6/2/02 → 6/6/02

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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Shu, H., Zhao, Y., Zhou, G., Chen, S., Wulfkuhle, J. D., Sgroi, D. C., Kruztsch, H., McLean, K., Knowlton, M., Sahin, A., Chen, Y., Petricoin, E., Steeg, P. S., & Zhao, Y. (2002). Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC). In Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics (pp. 227-228)

Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC). / Shu, Hongjun; Zhao, Yingxin; Zhou, Ge et al.
Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. 2002. p. 227-228.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Shu, H, Zhao, Y, Zhou, G, Chen, S, Wulfkuhle, JD, Sgroi, DC, Kruztsch, H, McLean, K, Knowlton, M, Sahin, A, Chen, Y, Petricoin, E, Steeg, PS & Zhao, Y 2002, Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC). in Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics. pp. 227-228, Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 6/2/02.

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title = "Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC)",

abstract = "A proteomic comparison of matched normal and cancer cells was presented, and the new facts of the transition of breast and esophageal cancer were revealed. Pure populations of normal and cancer cells were obtained using laser capture microdissection (LCM). Protein sample preparation, 2D-gel electrophoresis, and mass spectrometry of protein identification, and immunohistochemistry were performed. It was found that the 3D simulation of up and down-regulated protein spots. Results show the spot pairs of up-regulated fascin, equally expressed tubulin and down regulated annexin.",

author = "Hongjun Shu and Yingxin Zhao and Ge Zhou and She Chen and Wulfkuhle, \{Julia D.\} and Sgroi, \{Dennis C.\} and Henry Kruztsch and Kelly McLean and Melodie Knowlton and Aysegul Sahin and Yue Chen and Emanuel Petricoin and Steeg, \{Patricia S.\} and Yingming Zhao",

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language = "English (US)",

pages = "227--228",

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T1 - Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC)

AU - Shu, Hongjun

AU - Zhao, Yingxin

AU - Zhou, Ge

AU - Chen, She

AU - Wulfkuhle, Julia D.

AU - Sgroi, Dennis C.

AU - Kruztsch, Henry

AU - McLean, Kelly

AU - Knowlton, Melodie

AU - Sahin, Aysegul

AU - Chen, Yue

AU - Petricoin, Emanuel

AU - Steeg, Patricia S.

AU - Zhao, Yingming

PY - 2002/12/1

Y1 - 2002/12/1

N2 - A proteomic comparison of matched normal and cancer cells was presented, and the new facts of the transition of breast and esophageal cancer were revealed. Pure populations of normal and cancer cells were obtained using laser capture microdissection (LCM). Protein sample preparation, 2D-gel electrophoresis, and mass spectrometry of protein identification, and immunohistochemistry were performed. It was found that the 3D simulation of up and down-regulated protein spots. Results show the spot pairs of up-regulated fascin, equally expressed tubulin and down regulated annexin.

AB - A proteomic comparison of matched normal and cancer cells was presented, and the new facts of the transition of breast and esophageal cancer were revealed. Pure populations of normal and cancer cells were obtained using laser capture microdissection (LCM). Protein sample preparation, 2D-gel electrophoresis, and mass spectrometry of protein identification, and immunohistochemistry were performed. It was found that the 3D simulation of up and down-regulated protein spots. Results show the spot pairs of up-regulated fascin, equally expressed tubulin and down regulated annexin.

UR - https://www.scopus.com/pages/publications/2442710317

UR - https://www.scopus.com/pages/publications/2442710317#tab=citedBy

M3 - Conference contribution

AN - SCOPUS:2442710317

SP - 227

EP - 228

BT - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Identification and validation of aberrantly expressed proteins in breast ductal carcinoma in situ (DCIS) and esophageal squamous cell carcinoma (ESCC)

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