Identification and quantitative analysis of indole-3-acetyll-aspartate from seeds of Glycine max L.

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Abstract

Indole-3-acetyl-L-aspartate(IAAsp) was isolated from seeds of Glycine max L. cv. Hark and its identity established by its chromatographic performance and its mass spectral fragmentation. Following acid hydrolysis, the aspartate moiety was shown to be the L-enantiomer by reverse phase high performance liquid chromatographic retention time of the bisethyl ester derivatized with 2,3,4,6-tetra-O-acetyl-fl-D-glycopyranosyl isothiocyanate. Isotope dilution analysis using 114CIIAAsp as internal standard showed that soybean seed contained 10 pinol/kg IAAsp and this accounted for one-half of the total indoleacetic acid of the seed.

Original languageEnglish (US)
Pages (from-to)749-753
Number of pages5
JournalPlant physiology
Volume70
Issue number3
DOIs
StatePublished - 1982

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