Identification and elucidation of proline-rich antimicrobial peptides with enhanced potency and delivery

Pin Kuang Lai, Daniel T. Tresnak, Benjamin J. Hackel

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Proline-rich antimicrobial peptides (PrAMPs) kill bacteria via a nonlytic mechanism in which they permeate through the outer membrane, utilize protein-mediated transport across the inner membrane, and target the ribosome to inhibit protein synthesis. We previously reported that substitutions of oncocin (VDKPPYLPRPRPPRRIYNR–NH2) with a pair of cationic residues improved the antimicrobial activity. In this study, we applied the design protocol to three other PrAMPs: apidaecin-1b, pyrrhocoricin, and bactenecin 7(1–16) and found that the substitutions (R4K and I8K/R) for apidaecin-1b improve the activity by twofold (p <.05) against nonpathogenic Escherichia coli. Moreover, the substitutions (L7K/R and R14K) for pyrrhocoricin improve the activity by 2–10-fold (p <.05) against some strains of E. coli and Salmonella Typhimurium. We also performed activity tests against inner membrane protein (SbmA or YgdD) knockout strains. The result is consistent with previous studies that SbmA is the major transporter for apidaecin-1b and pyrrhocoricin derivatives. However, bactenecin 7(1–16) functions independently of these transporters. In addition, several apidaecin-1b derivatives exhibit enhanced activity relative to wild-type only in the absence of SbmA, which is consistent with mutations that enhance transport across the inner membrane. A high performance liquid chromatography-based kinetic assay for cellular association and internalization demonstrates that the selected cationic mutations can improve cellular association in minimal media, but this enhanced association is not required for increased activity, which suggests the importance of inner membrane transport. These functional studies on cationic mutants of PrAMPs advance understanding of potency and mechanism and advance the ability to engineer improved antimicrobials as evidenced by the identification of the pyrrhocoricin mutant (L7R and R14K) with 10-fold elevated potency against pathogenic E. coli.

Original languageEnglish (US)
Pages (from-to)2439-2450
Number of pages12
JournalBiotechnology and bioengineering
Volume116
Issue number10
DOIs
StatePublished - Oct 1 2019

Keywords

  • SbmA receptor
  • apidaecin-1b
  • bactenecin
  • proline-rich antimicrobial peptides
  • pyrrhocoricin

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural

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