Identification and dynamics of arabidopsis adaptor protein-2 complex and its involvement in floral organ development

Shohei Yamaoka, Yuki Shimono, Makoto Shirakawa, Yoichiro Fukao, Takashi Kawase, Noriyuki Hatsugai, Kentaro Tamura, Tomoo Shimada, Ikuko Hara-Nishimura

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

The adaptor protein-2 (AP-2) complex is a heterotetramer involved in clathrin-mediated endocytosis of cargo proteins from the plasma membrane in animal cells. The homologous genes of AP-2 subunits are present in the genomes of plants; however, their identities and roles in endocytic pathways are not clearly defined in plants. Here, we reveal the molecular composition of the AP-2 complex of Arabidopsis thaliana and its dynamics on the plasma membrane. We identified all of the α-, β-, σ-, and μ-subunits of the AP-2 complex and detected a weak interaction of the AP-2 complex with clathrin heavy chain. The m-subunit protein fused to green fluorescent protein (AP2M-GFP) was localized to the plasma membrane and to the cytoplasm. Live-cell imaging using a variable-angle epifluorescence microscope revealed that AP2M-GFP transiently forms punctate structures on the plasma membrane. Homozygous ap2m mutant plants exhibited abnormal floral structures, including reduced stamen elongation and delayed anther dehiscence, which led to a failure of pollination and a subsequent reduction of fertility. Our study provides a molecular basis for understanding AP-2-dependent endocytic pathways in plants and their roles in floral organ development and plant reproduction.

Original languageEnglish (US)
Pages (from-to)2958-2969
Number of pages12
JournalPlant Cell
Volume25
Issue number8
DOIs
StatePublished - Aug 2013

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