γ/δ T cells represent the major lymphocyte subset in calves, making the bovine system ideal to study the function of these T cells. In this report, we characterize 3 new anti-bovine y/5 Tcr antibodies. GD3.8, GD197, and GD3.1 recognized surface antigens on bovine g/d T cells with molecular weights of 45 kD or 80 kD under reducing or nonreducing conditions, respectively. These antibodies are specific for bovine y/8 Tcr. In preclearing and crossimmunoprecipitation experiments, using a previously published anti-y6 Ter mAb. GD3.8 recognized all y/S Tcrs; whereas GD3.1 and GD197 recognized mutually exclusive as well as overlapping subsets. All three antibodies are IgGls and work well in flow cytometric, immunoprecipitation, and immunohistological analysis. Preliminary surveys of normal organs using GD3.8 has shown that earlier predictions of the number of g/d T cells in tissues were underestimates. In the blood of healthy animals, the percentage of GD 197 positive cells always exceeded the percentage of 3.1-positive cells. The accumulation of γ/δ T cell subsets defined by their Tcr usage into sites of acute and chronic inflammation is currently being studied.
|Original language||English (US)|
|State||Published - Dec 1 1996|