Identification and characterization of cytopathogenic Mycoplasma hyorhinis from swine farms with a history of abortions

Jin Ho Shin, Han Soo Joo, Won Hyung Lee, Ho Bong Seok, Maria Calsamig, Carlos Pijoan, Thomas W. Molitor

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A virus-like cytopathic agent isolated from swine farms with a history of recurrent abortion episodes was investigated. We employed a differential display reverse transcription-polymerase chain reaction (ddRT-PCR) to obtain genetic information of the cytopathic agent. Partial nucleotide sequence (527 bp) obtained from differentially displayed PCR fragments showed 88.7% similarity with the 23S rRNA gene of Mycoplasma hyopneumoniae. Unexpectedly, the 5′ portion (1-333 bp) of the sequence shared 96.1% similarity with 5′ untranslated region (UTR) of human prostate tumor inducing gene 1 (PTI-1). Cytopathic effects and extranuclear DNA fluorescence were no longer observed when BM-cyclin was added in the culture medium, suggesting that BM-cyclin sensitive mycoplasma-like organisms caused the cell death. Further evidence supporting the cytopathic agent as a mycoplasma-like organism was obtained by the capability of 3H-thymidine and 3H-uridine incorporation, a single peak in buoyant density gradient profile (1.20-1.24 g/ml), and ultrastructural morphology. Unlike M. hyopneumoniae, the organism was not propagated in Friis medium. Nucleotide sequence of 16S rRNA obtained from the cytopathic agent showed 0.8-1.0% divergences with other M. hyorhinis strains, suggesting that the newly isolated cytopathogenic swine mycoplasma was a variant form of M. hyorhinis. Striking homology between a portion of the 23S rRNA gene of M. hyorhinis and 5′ UTR of human PTI-1 implicated that M. hyorhinis might potentially be related to the evolution of human PTI-1.

Original languageEnglish (US)
Pages (from-to)501-509
Number of pages9
JournalJournal of Veterinary Medical Science
Volume65
Issue number4
DOIs
StatePublished - Jan 1 2003

Fingerprint

Mycoplasma hyorhinis
abortion (animals)
Induced Abortion
Mycoplasma
Swine
Mycoplasma hyopneumoniae
prostatic neoplasms
Prostate
farms
swine
5' Untranslated Regions
rRNA Genes
5' untranslated regions
Phytoplasma
cyclins
ribosomal RNA
genes
Genes
Habitual Abortion
Neoplasms

Keywords

  • Differential display
  • Genetic identification
  • Mycoplasma hyorhinis

Cite this

Identification and characterization of cytopathogenic Mycoplasma hyorhinis from swine farms with a history of abortions. / Shin, Jin Ho; Joo, Han Soo; Lee, Won Hyung; Seok, Ho Bong; Calsamig, Maria; Pijoan, Carlos; Molitor, Thomas W.

In: Journal of Veterinary Medical Science, Vol. 65, No. 4, 01.01.2003, p. 501-509.

Research output: Contribution to journalArticle

Shin, Jin Ho ; Joo, Han Soo ; Lee, Won Hyung ; Seok, Ho Bong ; Calsamig, Maria ; Pijoan, Carlos ; Molitor, Thomas W. / Identification and characterization of cytopathogenic Mycoplasma hyorhinis from swine farms with a history of abortions. In: Journal of Veterinary Medical Science. 2003 ; Vol. 65, No. 4. pp. 501-509.
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