Identification and characterization of a growth factor secreted by an established cell line of human retinoblastoma maintained in serum-free medium

Norman A. Rubin, Joseph F. Tarsio, Andrew C. Borthwick, Dale S. Gregerson, Ted W. Reid

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A growth factor is obtained from a human retinoblastoma cell line (Y-79) growing in culture in the absence of any added serum or hormone. The retinoblastoma derived growth factor (RDGF) is still secreted by the Y-79 cells after they have been growing in a serum-free environment for over 5 months. RDGF stimulates both cell division and thymidine uptake of Swiss mouse 3T3 fibroblasts in media containing 0.2% fetal bovine serum. The thymidine uptake occurs maximally 16-42 hr after addition of RDGF. The factor has an apparent molecular weight of 38,000 as determined by Sephadex G-100 chromatography and is heat and trypsin labile. There appears to be additional material of higher MW which also stimulates thymidine uptake by the 3T3 cells.

Original languageEnglish (US)
Pages (from-to)105-112
Number of pages8
JournalVision Research
Volume21
Issue number1
DOIs
StatePublished - 1981

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