Identification and characterization of a growth factor secreted by an established cell line of human retinoblastoma maintained in serum-free medium

Norman A. Rubin; Joseph F. Tarsio; Andrew C. Borthwick; Dale S. Gregerson; Ted W. Reid

doi:10.1016/0042-6989(81)90142-5

Identification and characterization of a growth factor secreted by an established cell line of human retinoblastoma maintained in serum-free medium

Norman A. Rubin, Joseph F. Tarsio, Andrew C. Borthwick, Dale S. Gregerson, Ted W. Reid

Ophthalmology & Visual Neurosciences

Research output: Contribution to journal › Article › peer-review

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Abstract

A growth factor is obtained from a human retinoblastoma cell line (Y-79) growing in culture in the absence of any added serum or hormone. The retinoblastoma derived growth factor (RDGF) is still secreted by the Y-79 cells after they have been growing in a serum-free environment for over 5 months. RDGF stimulates both cell division and thymidine uptake of Swiss mouse 3T3 fibroblasts in media containing 0.2% fetal bovine serum. The thymidine uptake occurs maximally 16-42 hr after addition of RDGF. The factor has an apparent molecular weight of 38,000 as determined by Sephadex G-100 chromatography and is heat and trypsin labile. There appears to be additional material of higher MW which also stimulates thymidine uptake by the 3T3 cells.

Original language	English (US)
Pages (from-to)	105-112
Number of pages	8
Journal	Vision Research
Volume	21
Issue number	1
DOIs	https://doi.org/10.1016/0042-6989(81)90142-5
State	Published - 1981

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title = "Identification and characterization of a growth factor secreted by an established cell line of human retinoblastoma maintained in serum-free medium",

abstract = "A growth factor is obtained from a human retinoblastoma cell line (Y-79) growing in culture in the absence of any added serum or hormone. The retinoblastoma derived growth factor (RDGF) is still secreted by the Y-79 cells after they have been growing in a serum-free environment for over 5 months. RDGF stimulates both cell division and thymidine uptake of Swiss mouse 3T3 fibroblasts in media containing 0.2% fetal bovine serum. The thymidine uptake occurs maximally 16-42 hr after addition of RDGF. The factor has an apparent molecular weight of 38,000 as determined by Sephadex G-100 chromatography and is heat and trypsin labile. There appears to be additional material of higher MW which also stimulates thymidine uptake by the 3T3 cells.",

author = "Rubin, {Norman A.} and Tarsio, {Joseph F.} and Borthwick, {Andrew C.} and Gregerson, {Dale S.} and Reid, {Ted W.}",

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T1 - Identification and characterization of a growth factor secreted by an established cell line of human retinoblastoma maintained in serum-free medium

AU - Rubin, Norman A.

AU - Tarsio, Joseph F.

AU - Borthwick, Andrew C.

AU - Gregerson, Dale S.

AU - Reid, Ted W.

PY - 1981

Y1 - 1981

N2 - A growth factor is obtained from a human retinoblastoma cell line (Y-79) growing in culture in the absence of any added serum or hormone. The retinoblastoma derived growth factor (RDGF) is still secreted by the Y-79 cells after they have been growing in a serum-free environment for over 5 months. RDGF stimulates both cell division and thymidine uptake of Swiss mouse 3T3 fibroblasts in media containing 0.2% fetal bovine serum. The thymidine uptake occurs maximally 16-42 hr after addition of RDGF. The factor has an apparent molecular weight of 38,000 as determined by Sephadex G-100 chromatography and is heat and trypsin labile. There appears to be additional material of higher MW which also stimulates thymidine uptake by the 3T3 cells.

AB - A growth factor is obtained from a human retinoblastoma cell line (Y-79) growing in culture in the absence of any added serum or hormone. The retinoblastoma derived growth factor (RDGF) is still secreted by the Y-79 cells after they have been growing in a serum-free environment for over 5 months. RDGF stimulates both cell division and thymidine uptake of Swiss mouse 3T3 fibroblasts in media containing 0.2% fetal bovine serum. The thymidine uptake occurs maximally 16-42 hr after addition of RDGF. The factor has an apparent molecular weight of 38,000 as determined by Sephadex G-100 chromatography and is heat and trypsin labile. There appears to be additional material of higher MW which also stimulates thymidine uptake by the 3T3 cells.

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Identification and characterization of a growth factor secreted by an established cell line of human retinoblastoma maintained in serum-free medium

Abstract

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