Identification and characterization of a fast-neutron-induced mutant with elevated seed protein content in soybean

Elizabeth M. Prenger, Alexandra Ostezan, M. A.Rouf Mian, Robert M Stupar, Travis Glenn, Zenglu Li

Research output: Contribution to journalArticle

Abstract

Key message: Protein content of soybean is critical for utility of soybean meal. A fast-neutron-induced deletion on chromosome 12 was found to be associated with increased protein content. Abstract: Soybean seed composition affects the utility of soybean, and improving seed composition is an essential breeding goal. Fast neutron radiation introduces genomic mutations resulting in novel variation for traits of interest. Two elite soybean lines were irradiated with fast neutrons and screened for altered seed composition. Twenty-three lines with altered protein, oil, or sucrose content were selected based on near-infrared spectroscopy data from five environments and yield tested at five locations. Mutants with significantly increased protein averaged 19.1–36.8 g kg−1 more protein than the parents across 10 environments. Comparative genomic hybridization (CGH) identified putative mutations in a mutant, G15FN-12, that has 36.8 g kg−1 higher protein than the parent genotype, and whole genome sequencing (WGS) of the mutant has confirmed these mutations. An F2:3 population was developed from G15FN-12 to determine association between genomic changes and increased protein content. Bulked segregant analysis of the population using the SoySNP50K BeadChip identified a CGH- and WGS-confirmed deletion on chromosome 12 to be responsible for elevated protein content. The population was genotyped using a KASP marker designed at the mutation region, and significant association (P < 0.0001) between the deletion on chromosome 12 and elevated protein content was observed and confirmed in the F3:4 generation. The F2 segregants homozygous for the deletion averaged 27 g kg−1 higher seed protein and 8 g kg−1 lower oil than homozygous wild-type segregants. Mutants with altered seed composition are a new resource for gene function studies and provide elite materials for genetic improvement of seed composition.

Original languageEnglish (US)
Pages (from-to)2965-2983
Number of pages19
JournalTheoretical and Applied Genetics
Volume132
Issue number11
DOIs
StatePublished - Nov 1 2019

Fingerprint

Fast Neutrons
Soybeans
Seeds
protein content
soybeans
mutants
seeds
comparative genomic hybridization
mutation
Chromosomes, Human, Pair 12
Proteins
chromosomes
proteins
Mutation
Comparative Genomic Hybridization
genomics
oils
genome
Oils
near-infrared spectroscopy

Keywords

  • Chromosome 12
  • Deletion
  • Fast neutrons
  • Mutant
  • Protein content
  • Soybeans

PubMed: MeSH publication types

  • Journal Article

Cite this

Identification and characterization of a fast-neutron-induced mutant with elevated seed protein content in soybean. / Prenger, Elizabeth M.; Ostezan, Alexandra; Mian, M. A.Rouf; Stupar, Robert M; Glenn, Travis; Li, Zenglu.

In: Theoretical and Applied Genetics, Vol. 132, No. 11, 01.11.2019, p. 2965-2983.

Research output: Contribution to journalArticle

Prenger, Elizabeth M. ; Ostezan, Alexandra ; Mian, M. A.Rouf ; Stupar, Robert M ; Glenn, Travis ; Li, Zenglu. / Identification and characterization of a fast-neutron-induced mutant with elevated seed protein content in soybean. In: Theoretical and Applied Genetics. 2019 ; Vol. 132, No. 11. pp. 2965-2983.
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AU - Prenger, Elizabeth M.

AU - Ostezan, Alexandra

AU - Mian, M. A.Rouf

AU - Stupar, Robert M

AU - Glenn, Travis

AU - Li, Zenglu

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AB - Key message: Protein content of soybean is critical for utility of soybean meal. A fast-neutron-induced deletion on chromosome 12 was found to be associated with increased protein content. Abstract: Soybean seed composition affects the utility of soybean, and improving seed composition is an essential breeding goal. Fast neutron radiation introduces genomic mutations resulting in novel variation for traits of interest. Two elite soybean lines were irradiated with fast neutrons and screened for altered seed composition. Twenty-three lines with altered protein, oil, or sucrose content were selected based on near-infrared spectroscopy data from five environments and yield tested at five locations. Mutants with significantly increased protein averaged 19.1–36.8 g kg−1 more protein than the parents across 10 environments. Comparative genomic hybridization (CGH) identified putative mutations in a mutant, G15FN-12, that has 36.8 g kg−1 higher protein than the parent genotype, and whole genome sequencing (WGS) of the mutant has confirmed these mutations. An F2:3 population was developed from G15FN-12 to determine association between genomic changes and increased protein content. Bulked segregant analysis of the population using the SoySNP50K BeadChip identified a CGH- and WGS-confirmed deletion on chromosome 12 to be responsible for elevated protein content. The population was genotyped using a KASP marker designed at the mutation region, and significant association (P < 0.0001) between the deletion on chromosome 12 and elevated protein content was observed and confirmed in the F3:4 generation. The F2 segregants homozygous for the deletion averaged 27 g kg−1 higher seed protein and 8 g kg−1 lower oil than homozygous wild-type segregants. Mutants with altered seed composition are a new resource for gene function studies and provide elite materials for genetic improvement of seed composition.

KW - Chromosome 12

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KW - Protein content

KW - Soybeans

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