Hypoxic pulmonary vasoconstriction: Redox regulation of O 2-sensitive K + channels by a mitochondrial O 2-sensor in resistance artery smooth muscle cells

Evangelos D. Michelakis, Bernard Thébaud, E. Kenneth Weir, Stephen L. Archer

Research output: Contribution to journalReview articlepeer-review

136 Scopus citations


Hypoxic pulmonary vasoconstriction (HPV) is a widely-conserved mechanism for matching ventilation and perfusion that optimizes systemic PO 2. HPV is elicited by moderate alveolar hypoxia through a mechanism that is intrinsic to the pulmonary circulation, particularly the resistance pulmonary arteries (PA), and is robust even in isolated perfused lungs. Although modulated by the endothelium, HPV persists in denuded PA rings and PA smooth muscle cells (PASMC). Beginning within seconds of hypoxia, HPV plateaus in minutes and persists for hours. During focal hypoxia (e.g. atelectasis), HPV is restricted to the vascular segments serving hypoxic lobes, and diverts blood to better-ventilated segments without causing pulmonary hypertension (PHT). However, with global hypoxia, as occurs at high altitude or in the fetal lung, HPV increases pulmonary vascular resistance (PVR) and may contribute to PHT. This review focuses on a comprehensive Redox Theory of HPV but considers relevant modulatory factors (endothelin), triggering stimuli (cyclic ADP-ribose-induced release of sarcoplasmic reticulum (SR) Ca 2+) and sustaining pathways (Rho kinase-modulated Ca 2+ sensitization of the contractile apparatus). The Redox Theory proposes that an O 2-sensor in resistance PASMC (complexes I and III of the mitochondrial electron transport chain (ETC)) generates reactive O 2 species (ROS) in proportion to PO 2. During normoxia, a redox mediator, like hydrogen peroxide (H 2O 2), maintains voltage-gated O 2-sensitive K + channels (Kv) in an oxidized open state. Hypoxic withdrawal of ROS inhibits Kv channels, thereby depolarizing PASMCs, activating L-type voltage-gated Ca 2+ channels, enhancing Ca 2+ influx and promoting vasoconstriction. The role of O 2-sensitive K + channels is conserved in most specialized O 2-sensitive tissues, including the ductus arteriosus and carotid body. The unique occurrence of hypoxic vasoconstriction in the pulmonary circulation relates to the colocalization of an O 2-sensor and O 2-sensitive Kv channels in resistance PAs. HPV has relevance to human physiology, pathophysiology (high altitude pulmonary edema (HAPE) and PHT) and therapy (single lung anesthesia).

Original languageEnglish (US)
Pages (from-to)1119-1136
Number of pages18
JournalJournal of Molecular and Cellular Cardiology
Issue number6
StatePublished - Dec 2004

Bibliographical note

Funding Information:
Dr. Michelakis, Dr. Thébaud and Dr. Archer are supported by the Canada Foundation for Innovation, the Alberta Heart and Stroke Foundation (H&SF), the Alberta Heritage Foundation for Medical Research (AHFMR) and the Canadian Institutes for Health Research (CIHR). Drs Thébaud, Michelakis and Archer are supported by the Alberta Cardiovascular and Stroke Research Centre (ABACUS). Dr. Archer is supported by NIH-RO1-HL071115. E.K. Weir is supported by VA Merit Review Funding and National Heart, Lung and Blood Institute Grant RO1 HL-65322.


  • Cyclic-ADP ribose
  • Endothelin
  • Kv1.5
  • NAPDH oxidase
  • O sensing
  • Persistent PHT of the newborn
  • Pulmonary hypertension
  • Reactive O species
  • Rho kinase


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