Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles

Kevin Seekell, Matthew J. Crow, Stella Marinakos, Julie H Ostrander, Ashutosh Chilkoti, Adam Wax

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

This work presents simultaneous imaging and detection of three different cell receptors using three types of plasmonic nanoparticles (NPs). The size, shape, and composition-dependent scattering profiles of these NPs allow for a system of multiple distinct molecular markers using a single optical source. With this goal in mind, tags consisting of anti-epidermal growth factor receptor gold nanorods, anti-insulin-like growth factor 1-R silver nanospheres, and human epidermal growth factor receptor 2Ab gold nanospheres were developed to monitor the expression of receptors commonly overexpressed by cancer cells. These labels were chosen because they scatter strongly in distinct spectral windows. A hyperspectral darkfield microspectroscopy system was developed to record the scattering spectra of cells labeled with these molecular tags. Simultaneous monitoring of multiple tags may lead to applications such as profiling of cell line immunophenotype and investigation of receptor signaling pathways. Single, dual, and triple tag experiments were performed to analyze NP tag specificity as well as their interactions. Distinct resonance peaks were observed in these studies, showing the ability to characterize cell lines using conjugated NPs. However, interpreting shifts in these peaks due to changes in a cellular dielectric environment may be complicated by plasmon coupling between NPs bound to proximal receptors and other coupling mechanisms due to the receptors themselves.

Original languageEnglish (US)
Article number116003
JournalJournal of biomedical optics
Volume16
Issue number11
DOIs
StatePublished - Nov 1 2011

Fingerprint

Molecular imaging
Nanoparticles
nanoparticles
Cells
Nanospheres
Epidermal Growth Factor Receptor
Gold
Scattering
cultured cells
Insulin
Somatomedins
Nanorods
gold
Silver
insulin
Light sources
Labels
Hyperspectral imaging
scattering
markers

Keywords

  • cancer cells
  • immunophenotype
  • molecular imaging
  • multiplexing
  • nanoparticles
  • plasmonics

Cite this

Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles. / Seekell, Kevin; Crow, Matthew J.; Marinakos, Stella; Ostrander, Julie H; Chilkoti, Ashutosh; Wax, Adam.

In: Journal of biomedical optics, Vol. 16, No. 11, 116003, 01.11.2011.

Research output: Contribution to journalArticle

Seekell, Kevin ; Crow, Matthew J. ; Marinakos, Stella ; Ostrander, Julie H ; Chilkoti, Ashutosh ; Wax, Adam. / Hyperspectral molecular imaging of multiple receptors using immunolabeled plasmonic nanoparticles. In: Journal of biomedical optics. 2011 ; Vol. 16, No. 11.
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