Hyperperfusion and cardioplegia effects on myocardial high-energy phosphate distribution and energy expenditure

J. Zhang, L. Shorr, M. Yoshiyama, H. Merkle, Michael Garwood, D. C. Homans, R. J. Bache, Kamil Ugurbil, A. H.L. From

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8 Scopus citations

Abstract

This study examines the hypothesis that high-energy phosphate (HEP) compound levels in unstimulated in vivo myocardium are defined by 1) the level of perfusion and 2) nonperfusion-dependent metabolic characteristics. This hypothesis was tested by determining 1) the effects of pharmacological hyperperfusion of functioning myocardium on transmural HEP compound distribution, contractile function, and myocardial oxygen consumption rate (MV̇O2) as well as 2) the effects of KCl cardioplegia on transmural myocardial HEP compound distribution. Creatine phosphate (CP) and ATP were measured across the anterior left ventricular wall using spatially localized 31P-nuclear magnetic resonance (NMR). At baseline, the CP-to-ATP (CP/ATP) ratio was significantly lower in the subendocardium than in the subepicardium. This transmural HEP gradient was abolished by hyperperfusion without significant effects on contractile function or MV̇O2. Similarly, KCl arrest significantly increased CP and CP/ATP in all myocardial layers, and the transmural gradient of CP/ATP was abolished again. These studies indicate that in present experimental model 1) myocardial performance is not constrained by inadequate perfusion in any myocardial layer although modest oxygen limitation affects the kinetics of oxidative phosphorylation in the inner myocardial layers and 2) in all myocardial layers, submaximal activation of intermediary metabolism and oxidative phosphorylation reactions results in lower steady-state CP and higher ADP levels relative to their respective values when energy expenditure is markedly reduced by KCl arrest.

Original languageEnglish (US)
Pages (from-to)H894-H904
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume267
Issue number3 36-3
DOIs
StatePublished - 1994

Keywords

  • adenosine 5'-triphosphate
  • contractile function
  • creatine phosphate
  • hyperperfusion
  • myocardial oxygen consumption rate
  • phosphorus-31 nuclear magnetic resonance spectroscopy
  • potassium chloride cardioplegia

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