Hyperbaric oxygen therapy sensitizes nimustine treatment for glioma in mice

Zhaohui Lu, Jiawei Ma, Bing Liu, Chungang Dai, Tao Xie, Xiaoyu Ma, Ming Li, Jun Dong, Qing Lan, Qiang Huang

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


Nimustine (ACNU) has antitumor activities in patients with malignant glioma. Hyperbaric oxygen (HBO) may enhance the efficacy of certain therapies that are hampered by the hypoxic microenvironment. We examined the combined effects of ACNU and HBO in a GFP transgenic nude mice bearing human glioma model. Mice inoculated with human glioma cells SU3 were randomly divided into the four groups: (A) the control group, (B) the HBOT (HBO therapy) group, (C) the ACNU group, and (D) the HBOT+ACNU group. Tumor size was measured at the indicated time intervals with a caliper; mice were sacrificed 28 days after treatment, and immunohistochemistry staining and western blot analysis were carried out. By the end of the trial, the tumor weights of groups A, B, C, and D were (P < 0.05), 6.03 ± 1.47, 4.13 ± 1.82 (P < 0.05), 2.39 ± 0.25 (P < 0.05), and 1.43 ± 0.38 (P < 0.01), respectively. The expressions of TNF-α, MMP9, HIF-α, VEGF, NF-κB, and IL-1β were associated with the infiltration of inflammatory cells and the inhibition rate of tumor cells. Hyperbaric oxygen therapy (HBOT) could inhibit glioma cell proliferation and inflammatory cell infiltration, and exert a sensitizing effect on ACNU therapy partially through enhancing oxygen pressure (PO2) in tumor tissues and lower expression levels of HIF-1α, TNF-α, IL-1β, VEGF, MMP9, and NF-κB.

Original languageEnglish (US)
Pages (from-to)3147-3155
Number of pages9
JournalCancer medicine
Issue number11
StatePublished - Nov 1 2016
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2016 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.


  • Chemotherapy
  • glioma stem cell
  • green fluorescence transgenic nude mice
  • hyperbaric oxygen therapy
  • immunity inflammation
  • transplantation model


Dive into the research topics of 'Hyperbaric oxygen therapy sensitizes nimustine treatment for glioma in mice'. Together they form a unique fingerprint.

Cite this