Hyperbaric oxygen and basic fibroblast growth factor promote growth of irradiated bone

Xin Wang, Ivan Ding, Huchen Xie, Timothy Wu, Nancy Wersto, Kundi Huang, Paul Okunieff

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Purpose: The goal of the current experiment is to test for protective effect of hyperbaric oxygen (HBO) and basic fibroblast growth factor (bFGF) on bone growth. Methods and Materials: Control C3H mice received hind leg irradiation at 0, 10, 20, or 30 Gy. HBO-treated groups received radiation 1, 5, or 9 weeks before beginning HBO. The remaining groups began bFGF ± HBO 1 or 5 weeks after 30 Gy. HBO treatments were given 5 days per week for 4 weeks at 2 ATA for 3 h/day. bFGF was given intravenously at 6 μg twice a week for 4 weeks. Results: HBO improved bone growth after radiation in the 10 and 20 Gy groups. At 18 weeks control tibia length discrepancy is 0.0, 4.2, 8.2, and 10.7% after 0, 10, 20, and 30 Gy, respectively. HBO beginning in week 1, 5, or 9 following 10 Gy decreased these discrepancies to 2.0% (p < 0.05), 1.8% (p < 0.05), and 2.4% (p < 0.05), respectively. After 20 Gy, HBO decreased these discrepancies to 7.0% (p = ns), 4.9% (p < 0.05), and 3.6% (p < 0.05), respectively. At 30 Gy, HBO alone had no effect on bone shortening. bFGF improved tibia length discrepancy with or without HBO. At 18 weeks length discrepancies were 6.5% (p < 0.05) and 7.3 (p < 0.05), and after bFGF alone were 6.8% (p < 0.05) and 7.3% (p < 0.05) for treatment beginning in week 1 or 5, respectively. Tibial growth at 18 and 33 weeks following radiation were similar. Conclusion: Radiation effects on bone growth can be significant reduced by HBO after 10 or 20 Gy, but not after 30 Gy. At 30 Gy bFGF still significantly reduced the degree of bone shortening, but HBO provided no added benefit to bFGF therapy.

Original languageEnglish (US)
Pages (from-to)189-196
Number of pages8
JournalInternational Journal of Radiation Oncology Biology Physics
Volume40
Issue number1
DOIs
StatePublished - Jan 1 1998

Fingerprint

Bone Development
fibroblasts
Fibroblast Growth Factor 2
bones
Oxygen
oxygen
tibia
Radiation
Tibia
radiation
Bone and Bones
Inbred C3H Mouse
Radiation Effects
radiation effects
mice
therapy
Leg

Keywords

  • Angiogenesis
  • Cytokine
  • FGF
  • Prevention
  • Toxicity

Cite this

Hyperbaric oxygen and basic fibroblast growth factor promote growth of irradiated bone. / Wang, Xin; Ding, Ivan; Xie, Huchen; Wu, Timothy; Wersto, Nancy; Huang, Kundi; Okunieff, Paul.

In: International Journal of Radiation Oncology Biology Physics, Vol. 40, No. 1, 01.01.1998, p. 189-196.

Research output: Contribution to journalArticle

Wang, Xin ; Ding, Ivan ; Xie, Huchen ; Wu, Timothy ; Wersto, Nancy ; Huang, Kundi ; Okunieff, Paul. / Hyperbaric oxygen and basic fibroblast growth factor promote growth of irradiated bone. In: International Journal of Radiation Oncology Biology Physics. 1998 ; Vol. 40, No. 1. pp. 189-196.
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abstract = "Purpose: The goal of the current experiment is to test for protective effect of hyperbaric oxygen (HBO) and basic fibroblast growth factor (bFGF) on bone growth. Methods and Materials: Control C3H mice received hind leg irradiation at 0, 10, 20, or 30 Gy. HBO-treated groups received radiation 1, 5, or 9 weeks before beginning HBO. The remaining groups began bFGF ± HBO 1 or 5 weeks after 30 Gy. HBO treatments were given 5 days per week for 4 weeks at 2 ATA for 3 h/day. bFGF was given intravenously at 6 μg twice a week for 4 weeks. Results: HBO improved bone growth after radiation in the 10 and 20 Gy groups. At 18 weeks control tibia length discrepancy is 0.0, 4.2, 8.2, and 10.7{\%} after 0, 10, 20, and 30 Gy, respectively. HBO beginning in week 1, 5, or 9 following 10 Gy decreased these discrepancies to 2.0{\%} (p < 0.05), 1.8{\%} (p < 0.05), and 2.4{\%} (p < 0.05), respectively. After 20 Gy, HBO decreased these discrepancies to 7.0{\%} (p = ns), 4.9{\%} (p < 0.05), and 3.6{\%} (p < 0.05), respectively. At 30 Gy, HBO alone had no effect on bone shortening. bFGF improved tibia length discrepancy with or without HBO. At 18 weeks length discrepancies were 6.5{\%} (p < 0.05) and 7.3 (p < 0.05), and after bFGF alone were 6.8{\%} (p < 0.05) and 7.3{\%} (p < 0.05) for treatment beginning in week 1 or 5, respectively. Tibial growth at 18 and 33 weeks following radiation were similar. Conclusion: Radiation effects on bone growth can be significant reduced by HBO after 10 or 20 Gy, but not after 30 Gy. At 30 Gy bFGF still significantly reduced the degree of bone shortening, but HBO provided no added benefit to bFGF therapy.",
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T1 - Hyperbaric oxygen and basic fibroblast growth factor promote growth of irradiated bone

AU - Wang, Xin

AU - Ding, Ivan

AU - Xie, Huchen

AU - Wu, Timothy

AU - Wersto, Nancy

AU - Huang, Kundi

AU - Okunieff, Paul

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Purpose: The goal of the current experiment is to test for protective effect of hyperbaric oxygen (HBO) and basic fibroblast growth factor (bFGF) on bone growth. Methods and Materials: Control C3H mice received hind leg irradiation at 0, 10, 20, or 30 Gy. HBO-treated groups received radiation 1, 5, or 9 weeks before beginning HBO. The remaining groups began bFGF ± HBO 1 or 5 weeks after 30 Gy. HBO treatments were given 5 days per week for 4 weeks at 2 ATA for 3 h/day. bFGF was given intravenously at 6 μg twice a week for 4 weeks. Results: HBO improved bone growth after radiation in the 10 and 20 Gy groups. At 18 weeks control tibia length discrepancy is 0.0, 4.2, 8.2, and 10.7% after 0, 10, 20, and 30 Gy, respectively. HBO beginning in week 1, 5, or 9 following 10 Gy decreased these discrepancies to 2.0% (p < 0.05), 1.8% (p < 0.05), and 2.4% (p < 0.05), respectively. After 20 Gy, HBO decreased these discrepancies to 7.0% (p = ns), 4.9% (p < 0.05), and 3.6% (p < 0.05), respectively. At 30 Gy, HBO alone had no effect on bone shortening. bFGF improved tibia length discrepancy with or without HBO. At 18 weeks length discrepancies were 6.5% (p < 0.05) and 7.3 (p < 0.05), and after bFGF alone were 6.8% (p < 0.05) and 7.3% (p < 0.05) for treatment beginning in week 1 or 5, respectively. Tibial growth at 18 and 33 weeks following radiation were similar. Conclusion: Radiation effects on bone growth can be significant reduced by HBO after 10 or 20 Gy, but not after 30 Gy. At 30 Gy bFGF still significantly reduced the degree of bone shortening, but HBO provided no added benefit to bFGF therapy.

AB - Purpose: The goal of the current experiment is to test for protective effect of hyperbaric oxygen (HBO) and basic fibroblast growth factor (bFGF) on bone growth. Methods and Materials: Control C3H mice received hind leg irradiation at 0, 10, 20, or 30 Gy. HBO-treated groups received radiation 1, 5, or 9 weeks before beginning HBO. The remaining groups began bFGF ± HBO 1 or 5 weeks after 30 Gy. HBO treatments were given 5 days per week for 4 weeks at 2 ATA for 3 h/day. bFGF was given intravenously at 6 μg twice a week for 4 weeks. Results: HBO improved bone growth after radiation in the 10 and 20 Gy groups. At 18 weeks control tibia length discrepancy is 0.0, 4.2, 8.2, and 10.7% after 0, 10, 20, and 30 Gy, respectively. HBO beginning in week 1, 5, or 9 following 10 Gy decreased these discrepancies to 2.0% (p < 0.05), 1.8% (p < 0.05), and 2.4% (p < 0.05), respectively. After 20 Gy, HBO decreased these discrepancies to 7.0% (p = ns), 4.9% (p < 0.05), and 3.6% (p < 0.05), respectively. At 30 Gy, HBO alone had no effect on bone shortening. bFGF improved tibia length discrepancy with or without HBO. At 18 weeks length discrepancies were 6.5% (p < 0.05) and 7.3 (p < 0.05), and after bFGF alone were 6.8% (p < 0.05) and 7.3% (p < 0.05) for treatment beginning in week 1 or 5, respectively. Tibial growth at 18 and 33 weeks following radiation were similar. Conclusion: Radiation effects on bone growth can be significant reduced by HBO after 10 or 20 Gy, but not after 30 Gy. At 30 Gy bFGF still significantly reduced the degree of bone shortening, but HBO provided no added benefit to bFGF therapy.

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