Hygiene-Eliciting Brood Semiochemicals as a Tool for Assaying Honey Bee (Hymenoptera: Apidae) Colony Resistance to Varroa (Mesostigmata: Varroidae)

K. Wagoner, J. G. Millar, J. Keller, J. Bello, P. Waiker, C. Schal, M. Spivak, O. Rueppell

Research output: Contribution to journalArticlepeer-review

Abstract

Despite numerous interventions, the ectoparasitic mite Varroa (Varroa destructor Anderson and Trueman [Mesostigmata: Varroidae]) and the pathogens it vectors remain a primary threat to honey bee (Apis mellifera Linnaeus [Hymenoptera: Apidae]) health. Hygienic behavior, the ability to detect, uncap, and remove unhealthy brood from the colony, has been bred for selectively for over two decades and continues to be a promising avenue for improved Varroa management. Although hygienic behavior is expressed more in Varroa-resistant colonies, hygiene does not always confer resistance to Varroa. Additionally, existing Varroa resistance selection methods trade efficacy for efficiency, because those achieving the highest levels of Varroa resistance can be time-consuming, and thus expensive and impractical for apicultural use. Here, we tested the hypothesis that hygienic response to a mixture of semiochemicals associated with Varroa-infested honey bee brood can serve as an improved tool for predicting colony-level Varroa resistance. In support of our hypothesis, we demonstrated that a mixture of the compounds (Z)-10-tritriacontene, (Z)-8-hentriacontene, (Z)-8-heptadecene, and (Z)-6-pentadecene triggers hygienic behavior in a two-hour assay, and that high-performing colonies (hygienic response to ≥60% of treated cells) have significantly lower Varroa infestations, remove significantly more introduced Varroa, and are significantly more likely to survive the winter compared to low-performing colonies (hygienic response to <60% of treated cells). We discuss the relative efficacy and efficiency of this assay for facilitating apiary management decisions and selection of Varroa-resistant honey bees, as well as the relevance of these findings to honey bee health, pollination services, and social insect communication.

Original languageEnglish (US)
Article number4
JournalJournal of Insect Science
Volume21
Issue number6
DOIs
StatePublished - Nov 1 2021

Bibliographical note

Funding Information:
We thank Katie Lee, Bob Danka, Jeff Hull (Triad Bee Supply), and Calvin Terry (Midnight Bee Supply) for their respective contributions to the content and completion of this work. This work was funded and made possible by the United States Department of Agriculture (National Institute for Food and Agriculture, grant number 2017-68004-26321) and the North Carolina Biotechnology Center (2016-TEG-1503).

Publisher Copyright:
© 2021 The Author(s). Published by Oxford University Press on behalf of Entomological Society of America.

Keywords

  • alkene
  • chemical communication
  • hygienic behavior
  • social immunity
  • unhealthy brood odor

PubMed: MeSH publication types

  • Journal Article

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