Hydrophilic interaction chromatography on amino-silica phases complements reversed-phase high-performance liquid chromatography and capillary electrophoresis for peptide analysis

Alan R. Oyler; Barbara L. Armstrong; Jessica Y. Cha; Marilyn X. Zhou; Qing Yang; Robin I. Robinson; Richard Dunphy; David J. Burinsky

doi:10.1016/0021-9673(95)00987-6

Hydrophilic interaction chromatography on amino-silica phases complements reversed-phase high-performance liquid chromatography and capillary electrophoresis for peptide analysis

Alan R. Oyler
, Barbara L. Armstrong
, Jessica Y. Cha
, Marilyn X. Zhou
, Qing Yang
, Robin I. Robinson
, Richard Dunphy
, David J. Burinsky

Chemistry & Biochemistry

Research output: Contribution to journal › Article › peer-review

112 Scopus citations

Abstract

Hydrophilic interaction chromatography (HILIC) on amine bonded-phase silica columns provides separations of peptides that are complementary to those obtained with reversed-phase HPLC and free solution capillary electrophoresis. This is illustrated with the peptide drug atosiban and nine diastereomers. Moreover, one of the HILIC methods was suitable for coupling with electrospray mass spectrometry.

Original language	English (US)
Pages (from-to)	378-383
Number of pages	6
Journal	Journal of Chromatography A
Volume	724
Issue number	1-2
DOIs	https://doi.org/10.1016/0021-9673(95)00987-6
State	Published - Feb 16 1996

Keywords

atosiban
capillary electrophoresis
diastereomer separation
hydrophilic interaction chromatography
liquid chromatography-mass spectrometry
peptides
stationary phases, LC

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10.1016/0021-9673(95)00987-6

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Oyler, A. R., Armstrong, B. L., Cha, J. Y., Zhou, M. X., Yang, Q., Robinson, R. I., Dunphy, R., & Burinsky, D. J. (1996). Hydrophilic interaction chromatography on amino-silica phases complements reversed-phase high-performance liquid chromatography and capillary electrophoresis for peptide analysis. Journal of Chromatography A, 724(1-2), 378-383. https://doi.org/10.1016/0021-9673(95)00987-6

Hydrophilic interaction chromatography on amino-silica phases complements reversed-phase high-performance liquid chromatography and capillary electrophoresis for peptide analysis. / Oyler, Alan R.; Armstrong, Barbara L.; Cha, Jessica Y. et al.
In: Journal of Chromatography A, Vol. 724, No. 1-2, 16.02.1996, p. 378-383.

Research output: Contribution to journal › Article › peer-review

Oyler, AR, Armstrong, BL, Cha, JY, Zhou, MX, Yang, Q, Robinson, RI, Dunphy, R & Burinsky, DJ 1996, 'Hydrophilic interaction chromatography on amino-silica phases complements reversed-phase high-performance liquid chromatography and capillary electrophoresis for peptide analysis', Journal of Chromatography A, vol. 724, no. 1-2, pp. 378-383. https://doi.org/10.1016/0021-9673(95)00987-6

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abstract = "Hydrophilic interaction chromatography (HILIC) on amine bonded-phase silica columns provides separations of peptides that are complementary to those obtained with reversed-phase HPLC and free solution capillary electrophoresis. This is illustrated with the peptide drug atosiban and nine diastereomers. Moreover, one of the HILIC methods was suitable for coupling with electrospray mass spectrometry.",

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AU - Oyler, Alan R.

AU - Armstrong, Barbara L.

AU - Cha, Jessica Y.

AU - Zhou, Marilyn X.

AU - Yang, Qing

AU - Robinson, Robin I.

AU - Dunphy, Richard

AU - Burinsky, David J.

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AB - Hydrophilic interaction chromatography (HILIC) on amine bonded-phase silica columns provides separations of peptides that are complementary to those obtained with reversed-phase HPLC and free solution capillary electrophoresis. This is illustrated with the peptide drug atosiban and nine diastereomers. Moreover, one of the HILIC methods was suitable for coupling with electrospray mass spectrometry.

KW - atosiban

KW - capillary electrophoresis

KW - diastereomer separation

KW - hydrophilic interaction chromatography

KW - liquid chromatography-mass spectrometry

KW - peptides

KW - stationary phases, LC

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JF - Journal of Chromatography A

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ER -

Hydrophilic interaction chromatography on amino-silica phases complements reversed-phase high-performance liquid chromatography and capillary electrophoresis for peptide analysis

Abstract

Keywords

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