Hydrophilic interaction chromatography on amino-silica phases complements reversed-phase high-performance liquid chromatography and capillary electrophoresis for peptide analysis

Alan R. Oyler, Barbara L. Armstrong, Jessica Y. Cha, Marilyn X. Zhou, Qing Yang, Robin I. Robinson, Richard Dunphy, David J. Burinsky

Research output: Contribution to journalArticlepeer-review

85 Scopus citations

Abstract

Hydrophilic interaction chromatography (HILIC) on amine bonded-phase silica columns provides separations of peptides that are complementary to those obtained with reversed-phase HPLC and free solution capillary electrophoresis. This is illustrated with the peptide drug atosiban and nine diastereomers. Moreover, one of the HILIC methods was suitable for coupling with electrospray mass spectrometry.

Original languageEnglish (US)
Pages (from-to)378-383
Number of pages6
JournalJournal of Chromatography A
Volume724
Issue number1-2
DOIs
StatePublished - Feb 16 1996

Keywords

  • atosiban
  • capillary electrophoresis
  • diastereomer separation
  • hydrophilic interaction chromatography
  • liquid chromatography-mass spectrometry
  • peptides
  • stationary phases, LC

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