Hydrogen/deuterium exchange on protein solutions containing nucleic acids: Utility of protamine sulfate

Anton Poliakov, Paul Jardine, Peter E. Prevelige

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


Obtaining global hydrogen/deuterium (H/D) exchange data on proteins is an important first step in amide proton exchange experiments. Important information such as the mode of exchange, the cooperativity of folding/unfolding reactions, and the effects of ligand binding can be readily obtained in global exchange experiments. Many interesting biological systems are complexes containing both proteins and nucleic acids. The low pH conditions required to quench H/D exchange reactions result in the formation of stable protein/nucleic acid precipitates which interfere with the liquid chromatography step of the experiment and preclude obtaining mass spectrometric data. In this work we show that the precipitation of proteins and nucleic acids is electrostatic in nature and can be prevented by high ionic strength and by removing nucleic acids by protamine sulfate. Using protamine sulfate in quenching solution, we were able to obtain global H/D data with protein samples containing large amounts of DNA or RNA.

Original languageEnglish (US)
Pages (from-to)2423-2428
Number of pages6
JournalRapid Communications in Mass Spectrometry
Issue number16
StatePublished - Aug 2008


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