Hydrogen production using amino acids obtained by protein degradation in waste biomass by combined dark- and photo-fermentation

Jun Cheng; Lingkan Ding; Ao Xia; Richen Lin; Yuyou Li; Junhu Zhou; Kefa Cen

doi:10.1016/j.biortech.2014.11.109

Hydrogen production using amino acids obtained by protein degradation in waste biomass by combined dark- and photo-fermentation

Jun Cheng, Lingkan Ding, Ao Xia, Richen Lin, Yuyou Li, Junhu Zhou, Kefa Cen

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

The biological hydrogen production from amino acids obtained by protein degradation was comprehensively investigated to increase heating value conversion efficiency. The five amino acids (i.e., alanine, serine, aspartic acid, arginine, and leucine) produced limited hydrogen (0.2-16.2mL/g) but abundant soluble metabolic products (40.1-84.0mM) during dark-fermentation. The carbon conversion efficiencies of alanine (85.3%) and serine (94.1%) during dark-fermentation were significantly higher than those of other amino acids. Residual dark-fermentation solutions treated with zeolite for NH₄⁺ removal were inoculated with photosynthetic bacteria to further produce hydrogen during photo-fermentation. The hydrogen yields of alanine and serine through combined dark- and photo-fermentation were 418.6 and 270.2mL/g, respectively. The heating value conversion efficiency of alanine to hydrogen was 25.1%, which was higher than that of serine (21.2%).

Original language	English (US)
Pages (from-to)	13-19
Number of pages	7
Journal	Bioresource Technology
Volume	179
DOIs	https://doi.org/10.1016/j.biortech.2014.11.109
State	Published - Mar 1 2015
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by the National Natural Science Foundation – China ( 51176163 , 51476141 ), National High Technology R&D Program – China ( 2012AA050101 ), International Sci. & Tech. Cooperation Program – China ( 2012DFG61770 ), Zhejiang Provincial Natural Science Foundation – China ( LR14E060002 ), Program for New Century Excellent Talents in University – China ( NCET-11-0446 ), Specialized Research Fund for the Doctoral Program of Higher Education – China ( 20110101110021 ), Science and Technology Project of Guangxi Province – China ( 1346011-1 ), and Program of Introducing Talents of Discipline to University – China ( B08026 ).

Publisher Copyright:
© 2014 Elsevier Ltd.

Keywords

Amino acid
Dark-fermentation
Hydrogen production
Photo-fermentation

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2014.11.109

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title = "Hydrogen production using amino acids obtained by protein degradation in waste biomass by combined dark- and photo-fermentation",

abstract = "The biological hydrogen production from amino acids obtained by protein degradation was comprehensively investigated to increase heating value conversion efficiency. The five amino acids (i.e., alanine, serine, aspartic acid, arginine, and leucine) produced limited hydrogen (0.2-16.2mL/g) but abundant soluble metabolic products (40.1-84.0mM) during dark-fermentation. The carbon conversion efficiencies of alanine (85.3%) and serine (94.1%) during dark-fermentation were significantly higher than those of other amino acids. Residual dark-fermentation solutions treated with zeolite for NH4+ removal were inoculated with photosynthetic bacteria to further produce hydrogen during photo-fermentation. The hydrogen yields of alanine and serine through combined dark- and photo-fermentation were 418.6 and 270.2mL/g, respectively. The heating value conversion efficiency of alanine to hydrogen was 25.1%, which was higher than that of serine (21.2%).",

keywords = "Amino acid, Dark-fermentation, Hydrogen production, Photo-fermentation",

author = "Jun Cheng and Lingkan Ding and Ao Xia and Richen Lin and Yuyou Li and Junhu Zhou and Kefa Cen",

note = "Funding Information: This study was supported by the National Natural Science Foundation – China ( 51176163 , 51476141 ), National High Technology R&D Program – China ( 2012AA050101 ), International Sci. & Tech. Cooperation Program – China ( 2012DFG61770 ), Zhejiang Provincial Natural Science Foundation – China ( LR14E060002 ), Program for New Century Excellent Talents in University – China ( NCET-11-0446 ), Specialized Research Fund for the Doctoral Program of Higher Education – China ( 20110101110021 ), Science and Technology Project of Guangxi Province – China ( 1346011-1 ), and Program of Introducing Talents of Discipline to University – China ( B08026 ). Publisher Copyright: {\textcopyright} 2014 Elsevier Ltd.",

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AU - Cheng, Jun

AU - Ding, Lingkan

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AU - Lin, Richen

AU - Li, Yuyou

AU - Zhou, Junhu

AU - Cen, Kefa

N1 - Funding Information: This study was supported by the National Natural Science Foundation – China ( 51176163 , 51476141 ), National High Technology R&D Program – China ( 2012AA050101 ), International Sci. & Tech. Cooperation Program – China ( 2012DFG61770 ), Zhejiang Provincial Natural Science Foundation – China ( LR14E060002 ), Program for New Century Excellent Talents in University – China ( NCET-11-0446 ), Specialized Research Fund for the Doctoral Program of Higher Education – China ( 20110101110021 ), Science and Technology Project of Guangxi Province – China ( 1346011-1 ), and Program of Introducing Talents of Discipline to University – China ( B08026 ). Publisher Copyright: © 2014 Elsevier Ltd.

PY - 2015/3/1

Y1 - 2015/3/1

N2 - The biological hydrogen production from amino acids obtained by protein degradation was comprehensively investigated to increase heating value conversion efficiency. The five amino acids (i.e., alanine, serine, aspartic acid, arginine, and leucine) produced limited hydrogen (0.2-16.2mL/g) but abundant soluble metabolic products (40.1-84.0mM) during dark-fermentation. The carbon conversion efficiencies of alanine (85.3%) and serine (94.1%) during dark-fermentation were significantly higher than those of other amino acids. Residual dark-fermentation solutions treated with zeolite for NH4+ removal were inoculated with photosynthetic bacteria to further produce hydrogen during photo-fermentation. The hydrogen yields of alanine and serine through combined dark- and photo-fermentation were 418.6 and 270.2mL/g, respectively. The heating value conversion efficiency of alanine to hydrogen was 25.1%, which was higher than that of serine (21.2%).

AB - The biological hydrogen production from amino acids obtained by protein degradation was comprehensively investigated to increase heating value conversion efficiency. The five amino acids (i.e., alanine, serine, aspartic acid, arginine, and leucine) produced limited hydrogen (0.2-16.2mL/g) but abundant soluble metabolic products (40.1-84.0mM) during dark-fermentation. The carbon conversion efficiencies of alanine (85.3%) and serine (94.1%) during dark-fermentation were significantly higher than those of other amino acids. Residual dark-fermentation solutions treated with zeolite for NH4+ removal were inoculated with photosynthetic bacteria to further produce hydrogen during photo-fermentation. The hydrogen yields of alanine and serine through combined dark- and photo-fermentation were 418.6 and 270.2mL/g, respectively. The heating value conversion efficiency of alanine to hydrogen was 25.1%, which was higher than that of serine (21.2%).

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KW - Hydrogen production

KW - Photo-fermentation

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Hydrogen production using amino acids obtained by protein degradation in waste biomass by combined dark- and photo-fermentation

Abstract

Bibliographical note

Keywords

UN SDGs

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