Hyaluronic acid-siRNA conjugate/reducible polyethylenimine complexes for targeted siRNA delivery

Yeon Lim Jang, Sook Hee Ku, So Jin Lee, Jae Hyung Park, Won Jong Kim, Ick Chan Kwon, Sun Hwa Kim, Ji Hoon Jeong

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

The clinical applications of therapeutic siRNA remain as a challenge due to the lack of efficient delivery system. In the present study, hyaluronic acid-siRNA conjugate (HA-SS-siRNA)/reducible polyethylenimine (BPEI1.2k-SS) complexes were developed to efficiently deliver the siRNA to HA receptor abundant region with the improved siRNA stability. HA and siRNA were conjugated with disulfide bonds, which are cleavable in cytoplasm. The synthesized HA-SS-siRNA was further complexed with BPEI1.2k-SS, resulting in the formation of spherical nanostructures with approximately 190 nm of size and neutral surface charge. HA-SS-siRNA/BPEI1.2k-SS complexes exhibited the improved stability against serum proteins or polyanions. These complexes were successfully translocated into intracellular region via HA receptor-mediated endocytosis, and silenced target gene expression.

Original languageEnglish (US)
Pages (from-to)7388-7394
Number of pages7
JournalJournal of Nanoscience and Nanotechnology
Volume14
Issue number10
DOIs
StatePublished - Oct 1 2014

Keywords

  • Hyaluronic acid
  • Reducible polyethylenimine
  • Tumor targeting
  • siRNA delivery

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    Jang, Y. L., Ku, S. H., Lee, S. J., Park, J. H., Kim, W. J., Kwon, I. C., Kim, S. H., & Jeong, J. H. (2014). Hyaluronic acid-siRNA conjugate/reducible polyethylenimine complexes for targeted siRNA delivery. Journal of Nanoscience and Nanotechnology, 14(10), 7388-7394. https://doi.org/10.1166/jnn.2014.9583