Hyaluronan stimulates ex vivo B lymphocyte chemotaxis and cytokine production in a murine model of fungal allergic asthma

Sumit Ghosh, Scott A. Hoselton, Steve B. Wanjara, Jennifer Carlson, James B. McCarthy, Glenn P. Dorsam, Jane M. Schuh

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Allergic asthma is a chronic inflammatory disease of the airways characterized by excessive eosinophilic and lymphocytic inflammation with associated changes in the extracellular matrix (ECM) resulting in airway wall remodeling. Hyaluronan (HA) is a nonsulfated glycosaminoglycan ECM component that functions as a structural cushion in its high molecular mass (HMM) but has been implicated in metastasis and other disease processes when it is degraded to smaller fragments. However, relatively little is known about the role HA in mediating inflammatory responses in allergy and asthma. In the present study, we used a murine Aspergillus fumigatus inhalational model to mimic human disease. After observing in vivo that a robust B cell recruitment followed a massive eosinophilic egress to the lumen of the allergic lung and corresponded with the detection of low molecular mass HA (LMM HA), we examined the effect of HA on B cell chemotaxis and cytokine production in the ex vivo studies. We found that LMM HA functioned through a CD44-mediated mechanism to elicit chemotaxis of B lymphocytes, while high molecular mass HA (HMM HA) had little effect. LMM HA, but not HMM HA, also elicited the production of IL-10 and TGF-β1 in these cells. Taken together, these findings demonstrate a critical role for ECM components in mediating leukocyte migration and function which are critical to the maintenance of allergic inflammatory responses.

Original languageEnglish (US)
Pages (from-to)899-909
Number of pages11
Issue number7
StatePublished - Jul 1 2015

Bibliographical note

Funding Information:
These studies were funded by grants from the NIH ( 1R15HL117254-01 to J.M.S. and 1R15AI101968-01A1 to G.P.D.).

Publisher Copyright:
© 2015 Elsevier GmbH.

Copyright 2020 Elsevier B.V., All rights reserved.


  • Aspergillus fumigatus
  • B lymphocytes
  • Hyaluronan


Dive into the research topics of 'Hyaluronan stimulates ex vivo B lymphocyte chemotaxis and cytokine production in a murine model of fungal allergic asthma'. Together they form a unique fingerprint.

Cite this