TY - JOUR
T1 - Human influenza viral infection in utero increases nNOS expression in hippocampi of neonatal mice
AU - Fatemi, S H
AU - Sidwell, Robert
AU - Akhter, Pervez
AU - Sedgewick, Jerry
AU - Thuras, Paul
AU - Bailey, Kevin
AU - Kist, David
PY - 1998/5
Y1 - 1998/5
N2 - We investigated the role of maternal exposure to human influenza virus (HI) in C57BL/6 mice on day 9 of pregnancy on hippocampal expression of nNOS in day 0 neonates and compared that to sham-infected pups. Qualitative analysis using polyclonal antibody to nNOS showed overall increases in immunoreactivity (IR) in hippocampal and dentate layers of day 0 infected neonates when compared to sham-infected animals. These increases in nNOS immunoreactivity were pronounced in hippocampal plate, intermediate, molecular, subplate, and dentate areas. Quantitative analysis of specific immunogold silver-enhanced nNOS IR via densitometry showed nNOS IR increases of 26-71.6% in all layers, i.e., hippocampal plate (35.1%), dentate area (71.6%), molecular area (43.75%), subplate (45.7%), and intermediate zone (26%) in infected neonatal brains vs. controls. The changes in levels of nNOS expression in hippocampi of neonates born to mothers exposed to HI virus during the second trimester of pregnancy may reflect the potential for glutamatergic excitotoxicity via activation of NMDA receptors in the developing brains of these neonatal mice.
AB - We investigated the role of maternal exposure to human influenza virus (HI) in C57BL/6 mice on day 9 of pregnancy on hippocampal expression of nNOS in day 0 neonates and compared that to sham-infected pups. Qualitative analysis using polyclonal antibody to nNOS showed overall increases in immunoreactivity (IR) in hippocampal and dentate layers of day 0 infected neonates when compared to sham-infected animals. These increases in nNOS immunoreactivity were pronounced in hippocampal plate, intermediate, molecular, subplate, and dentate areas. Quantitative analysis of specific immunogold silver-enhanced nNOS IR via densitometry showed nNOS IR increases of 26-71.6% in all layers, i.e., hippocampal plate (35.1%), dentate area (71.6%), molecular area (43.75%), subplate (45.7%), and intermediate zone (26%) in infected neonatal brains vs. controls. The changes in levels of nNOS expression in hippocampi of neonates born to mothers exposed to HI virus during the second trimester of pregnancy may reflect the potential for glutamatergic excitotoxicity via activation of NMDA receptors in the developing brains of these neonatal mice.
KW - Hippocampus
KW - Human influenza virus
KW - Mice
KW - Schizophrenia
KW - Second trimester of pregnancy
KW - nNOS
UR - http://www.scopus.com/inward/record.url?scp=0031970085&partnerID=8YFLogxK
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U2 - 10.1002/(SICI)1098-2396(199805)29:1<84::AID-SYN8>3.0.CO;2-7
DO - 10.1002/(SICI)1098-2396(199805)29:1<84::AID-SYN8>3.0.CO;2-7
M3 - Article
C2 - 9552178
AN - SCOPUS:0031970085
SN - 0887-4476
VL - 29
SP - 84
EP - 88
JO - Synapse
JF - Synapse
IS - 1
ER -