Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells

Andrielle Castilho-Fernandes, Danilo Candido de Almeida, Aparecida Maria Fontes, Fernanda Ursoli Ferreira Melo, Virgínia Picanço-Castro, Marcela Cristina Freitas, Maristela D. Orellana, Patricia V.B. Palma, Perry B. Hackett, Scott L. Friedman, Dimas Tadeu Covas

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Abstract

The LX-2 cell line has characteristics of hepatic stellate cells (HSCs), which are considered pericytes of the hepatic microcirculatory system. Recent studies have suggested that HSCs might have mesenchymal origin. We have performed an extensive characterization of the LX-2 cells and have compared their features with those of mesenchymal cells. Our data show that LX-2 cells have a phenotype resembling activated HSCs as well as bone marrow-derived mesenchymal stem cells (BM-MSCs). Our immunophenotypic analysis showed that LX-2 cells are positive for activated HSC markers (αSMA, GFAP, nestin and CD271) and classical mesenchymal makers (CD105, CD44, CD29, CD13, CD90, HLA class-I, CD73, CD49e, CD166 and CD146) but negative for the endothelial marker CD31 and endothelial progenitor cell marker CD133 as well as hematopoietic markers (CD45 and CD34). LX-2 cells also express the same transcripts found in immortalized and primary BM-MSCs (vimentin, annexin 5, collagen 1A, NG2 and CD140b), although at different levels. We show that LX-2 cells are capable to differentiate into multilineage mesenchymal cells in vitro and can stimulate new blood vessel formation in vivo. LX-2 cells appear not to possess tumorigenic potential. Thus, the LX-2 cell line behaves as a multipotent cell line with similarity to BM-MSCs. This line should be useful for further studies to elucidate liver regeneration mechanisms and be the foundation for development of hepatic cell-based therapies.

Original languageEnglish (US)
Pages (from-to)664-672
Number of pages9
JournalExperimental and Molecular Pathology
Volume91
Issue number3
DOIs
StatePublished - Dec 1 2011

Fingerprint

Hepatic Stellate Cells
Stem cells
Mesenchymal Stromal Cells
Bone
Bone Marrow
Cell Line
Cells
Annexins
Nestin
Endothelial cells
Blood vessels
Vimentin
Liver
Collagen
Pericytes
Liver Regeneration
Cell- and Tissue-Based Therapy
Blood Vessels
Hepatocytes
Phenotype

Keywords

  • Hepatic stellate cell line
  • LX-2
  • Mesenchymal stem cells
  • Pericytes

Cite this

Castilho-Fernandes, A., de Almeida, D. C., Fontes, A. M., Melo, F. U. F., Picanço-Castro, V., Freitas, M. C., ... Covas, D. T. (2011). Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells. Experimental and Molecular Pathology, 91(3), 664-672. https://doi.org/10.1016/j.yexmp.2011.09.002

Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells. / Castilho-Fernandes, Andrielle; de Almeida, Danilo Candido; Fontes, Aparecida Maria; Melo, Fernanda Ursoli Ferreira; Picanço-Castro, Virgínia; Freitas, Marcela Cristina; Orellana, Maristela D.; Palma, Patricia V.B.; Hackett, Perry B.; Friedman, Scott L.; Covas, Dimas Tadeu.

In: Experimental and Molecular Pathology, Vol. 91, No. 3, 01.12.2011, p. 664-672.

Research output: Contribution to journalArticle

Castilho-Fernandes, A, de Almeida, DC, Fontes, AM, Melo, FUF, Picanço-Castro, V, Freitas, MC, Orellana, MD, Palma, PVB, Hackett, PB, Friedman, SL & Covas, DT 2011, 'Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells', Experimental and Molecular Pathology, vol. 91, no. 3, pp. 664-672. https://doi.org/10.1016/j.yexmp.2011.09.002
Castilho-Fernandes, Andrielle ; de Almeida, Danilo Candido ; Fontes, Aparecida Maria ; Melo, Fernanda Ursoli Ferreira ; Picanço-Castro, Virgínia ; Freitas, Marcela Cristina ; Orellana, Maristela D. ; Palma, Patricia V.B. ; Hackett, Perry B. ; Friedman, Scott L. ; Covas, Dimas Tadeu. / Human hepatic stellate cell line (LX-2) exhibits characteristics of bone marrow-derived mesenchymal stem cells. In: Experimental and Molecular Pathology. 2011 ; Vol. 91, No. 3. pp. 664-672.
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AU - Melo, Fernanda Ursoli Ferreira

AU - Picanço-Castro, Virgínia

AU - Freitas, Marcela Cristina

AU - Orellana, Maristela D.

AU - Palma, Patricia V.B.

AU - Hackett, Perry B.

AU - Friedman, Scott L.

AU - Covas, Dimas Tadeu

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