Human GLTP and mutant forms of ACD11 suppress cell death in the Arabidopsis acd11 mutant

Nikolaj H.T. Petersen, Lea V. McKinney, Helen Pike, Daniel Hofius, Asif Zakaria, Peter Brodersen, Morten Petersen, Rhoderick E. Brown, John Mundy

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Abstract

The Arabidopsis acd11 mutant exhibits runaway, programmed cell death due to the loss of a putative sphingosine transfer protein (ACD11) with homology to mammalian GLTP. We demonstrate that transgenic expression in Arabidopsis thaliana of human GLTP partially suppressed the phenotype of the acd11 null mutant, resulting in delayed programmed cell death development and plant survival. Surprisingly, a GLTP mutant form impaired in glycolipid transfer activity also complemented the acd11 mutants. To understand the relationship between functional complementarity and transfer activity, we generated site-specific mutants in ACD11 based on homologous GLTP residues required for glycolipid transfer. We show that these ACD11 mutant forms are impaired in their in vitro transfer activity of sphingolipids. However, transgenic expression of these mutant forms fully complemented acd11 mutant cell death, and transgenic plants showed normal induction of hypersensitive cell death upon infection with avirulent strains of Pseudomonas syringae. The significance of these findings with respect to the function(s) of ACD11 in sphingolipid transport and cell death regulation is discussed.

Original languageEnglish (US)
Pages (from-to)4378-4388
Number of pages11
JournalFEBS Journal
Volume275
Issue number17
DOIs
StatePublished - Sep 1 2008

Cite this

Petersen, N. H. T., McKinney, L. V., Pike, H., Hofius, D., Zakaria, A., Brodersen, P., Petersen, M., Brown, R. E., & Mundy, J. (2008). Human GLTP and mutant forms of ACD11 suppress cell death in the Arabidopsis acd11 mutant. FEBS Journal, 275(17), 4378-4388. https://doi.org/10.1111/j.1742-4658.2008.06584.x