Human endothelial cell growth on mussel-inspired nanofiber scaffold for vascular tissue engineering

Sook Hee Ku, Chan Beum Park

Research output: Contribution to journalArticlepeer-review

285 Scopus citations

Abstract

The endothelialization of prosthetic scaffolds is considered to be an effective strategy to improve the effectiveness of small-diameter vascular grafts. We report the development of a nanofibrous scaffold that has a polymeric core and a shell mimicking mussel adhesive for enhanced attachment, proliferation, and phenotypic maintenance of human endothelial cells. Polycaprolactone (PCL) was chosen as a core material because of its good biodegradability and mechanical properties suitable for tissue engineering. PCL was electrospun into nanofibers with a diameter of approximately 700 nm and then coated with poly(dopamine) (PDA) to functionalize the surface of PCL nanofibers with numerous catechol moieties similar to mussel adhesives in nature. The formation of a PDA ad-layer was analyzed using multiple techniques, including scanning electron microscopy, Raman spectroscopy, and water contact angle measurements. When PDA-coated PCL nanofibers were compared to unmodified and gelatin-coated nanofibers, human umbilical vein endothelial cells (HUVECs) exhibited highly enhanced adhesion and viability, increased stress fiber formation, and positive expression of endothelial cell markers (e.g., PECAM-1 and vWF).

Original languageEnglish (US)
Pages (from-to)9431-9437
Number of pages7
JournalBiomaterials
Volume31
Issue number36
DOIs
StatePublished - Dec 2010

Bibliographical note

Funding Information:
This study was supported by the National Research Foundation (NRF) via National Research Laboratory (NRL) (R0A-2008-000-20041-0) and Converging Research Center (2009-0082276) programs. This research was also partially supported by the BioGreen 21 Program (20070301034038) and a research grant from the KAIST Institute for the NanoCentury (KINC), Republic of Korea .

Keywords

  • Electrospinning
  • Endothelial cells
  • Mussel adhesives
  • Nanofibers
  • Poly(dopamine)
  • Vascular tissue scaffold

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