Human cytomegalovirus immediate early proteins upregulate endothelial p53 function

Jun Wang, Paul H. Marker, John D. Belcher, David E.L. Wilcken, Linda J. Burns, Gregory M. Vercellotti, Xing L. Wang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Infected endothelial cells are found to be resistant to apoptosis possibly mediated by p53 cytoplasmic sequestration. We explored whether the immediate early 84 kDa protein (IE84) of cytomegalovirus (CMV) is responsible for p53 cytoplasmic sequestration. The endothelial cells were transfected with plasmids containing IE1 and 2 coding regions which are known to synthesize IE84 and 72 proteins. Our study found that p53 expression was significantly elevated in endothelial cells transfected with IE1 and 2 plasmids. However, p53 was only found in the nucleus rather than sequestered in the cytoplasm. We have demonstrated that IE84 and 72 are not responsible for p53 dysfunction caused by CMV infection, rather they upregulate p53 function and promote endothelial apoptosis. Copyright (C) 2000 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)213-216
Number of pages4
JournalFEBS Letters
Volume474
Issue number2-3
DOIs
StatePublished - Jun 2 2000

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Endothelial cells
Up-Regulation
Endothelial Cells
Plasmids
Apoptosis
Cytomegalovirus Infections
Cytomegalovirus
Cytoplasm
Proteins
cytomegalovirus immediate-early proteins

Keywords

  • Cytomegalovirus
  • Endothelial apoptosis
  • Immediate early gene
  • p53

Cite this

Human cytomegalovirus immediate early proteins upregulate endothelial p53 function. / Wang, Jun; Marker, Paul H.; Belcher, John D.; Wilcken, David E.L.; Burns, Linda J.; Vercellotti, Gregory M.; Wang, Xing L.

In: FEBS Letters, Vol. 474, No. 2-3, 02.06.2000, p. 213-216.

Research output: Contribution to journalArticle

Wang, Jun ; Marker, Paul H. ; Belcher, John D. ; Wilcken, David E.L. ; Burns, Linda J. ; Vercellotti, Gregory M. ; Wang, Xing L. / Human cytomegalovirus immediate early proteins upregulate endothelial p53 function. In: FEBS Letters. 2000 ; Vol. 474, No. 2-3. pp. 213-216.
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