Understanding the fine-scale genome sequence diversity that exists within natural populations is important for developing models of species migration, temporal stability, and range expansion. For invasive species, agricultural pests, and disease vectors, sequence diversity at specific loci in the genome can impact the efficacy of next-generation genetic biocontrol strategies. Here we describe a pipeline for haplotype-resolution genetic variant discovery and quantification from thousands of Spotted Wing Drosophila (Drosophila suzukii, SWD) isolated at two field sites in the North-Central United States (Minnesota) across two seasons. We observed highly similar single nucleotide polymorphism (SNP) frequencies at each genomic location at each field site and year. This supports the hypotheses that SWD overwinters in Minnesota, is annually populated by the same source populations or a combination of both theories. Also, the stable genetic structure of SWD populations allows for the rational design of genetic biocontrol technologies for population suppression.
Bibliographical noteFunding Information:
Funding for this project, including support to NF, was provided by the Minnesota Invasive Terrestrial Plants and Pests Center, through the Environment and Natural Resources Trust Fund, as recommended by the Legislative-Citizen Commission on Minnesota Resources (LCCMR). EB and DE were funded primarily by the Rapid Agricultural Response Fund, Award 0081527, via the Minnesota Agricultural Experiment Station, University of Minnesota.
Copyright © 2022 Feltman, Burkness, Ebbenga, Hutchison and Smanski.
- amplicon sequencing
- genetic biocontrol
- population genetics