Abstract
Transfer of H3-cerebroside sulfate (CS) from aqueous phase to nonaqueous phases (heptane interface) was studied in the absence and presence of opiates, cations and phosphatidylserine. The degree of H3-CS re-distribution was dependent on the concentration of these substances used. The concentration of an opiate agonist (GPA-1657) required to increase H3-CS by 50% in the nonaqueous phase was much lower than that of its corresponding antagonist (GPA-2163) and the value for calcium was 100 times less than sodium. Opiate antagonist (GPA-2163) and phosphatidylserine inhibited the agonist induced re-distribution of H3-CS. Thus, the data seem to indicate that the distribution of H3-CS between these two phases was determined by hydrophobic-hydropholic balance of H3-CS and this balance was dependent on the counter ion pairing with CS. This finding is consistent with our previous observation that opiate agonist-CS complex was more hydrophobic than free CS of the CS-complex formed with opiate antagonist.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 117-123 |
| Number of pages | 7 |
| Journal | Life Sciences |
| Volume | 19 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1 1976 |
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H3-cerebroside sulfate re-distribution induced by cation, opiate or phosphatidyl serine. / Tae Mook Cho, Mook Cho; Jung Sook Cho, Sook Cho; Loh, Horace H.
In: Life Sciences, Vol. 19, No. 1, 01.07.1976, p. 117-123.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - H3-cerebroside sulfate re-distribution induced by cation, opiate or phosphatidyl serine
AU - Tae Mook Cho, Mook Cho
AU - Jung Sook Cho, Sook Cho
AU - Loh, Horace H.
PY - 1976/7/1
Y1 - 1976/7/1
N2 - Transfer of H3-cerebroside sulfate (CS) from aqueous phase to nonaqueous phases (heptane interface) was studied in the absence and presence of opiates, cations and phosphatidylserine. The degree of H3-CS re-distribution was dependent on the concentration of these substances used. The concentration of an opiate agonist (GPA-1657) required to increase H3-CS by 50% in the nonaqueous phase was much lower than that of its corresponding antagonist (GPA-2163) and the value for calcium was 100 times less than sodium. Opiate antagonist (GPA-2163) and phosphatidylserine inhibited the agonist induced re-distribution of H3-CS. Thus, the data seem to indicate that the distribution of H3-CS between these two phases was determined by hydrophobic-hydropholic balance of H3-CS and this balance was dependent on the counter ion pairing with CS. This finding is consistent with our previous observation that opiate agonist-CS complex was more hydrophobic than free CS of the CS-complex formed with opiate antagonist.
AB - Transfer of H3-cerebroside sulfate (CS) from aqueous phase to nonaqueous phases (heptane interface) was studied in the absence and presence of opiates, cations and phosphatidylserine. The degree of H3-CS re-distribution was dependent on the concentration of these substances used. The concentration of an opiate agonist (GPA-1657) required to increase H3-CS by 50% in the nonaqueous phase was much lower than that of its corresponding antagonist (GPA-2163) and the value for calcium was 100 times less than sodium. Opiate antagonist (GPA-2163) and phosphatidylserine inhibited the agonist induced re-distribution of H3-CS. Thus, the data seem to indicate that the distribution of H3-CS between these two phases was determined by hydrophobic-hydropholic balance of H3-CS and this balance was dependent on the counter ion pairing with CS. This finding is consistent with our previous observation that opiate agonist-CS complex was more hydrophobic than free CS of the CS-complex formed with opiate antagonist.
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U2 - 10.1016/0024-3205(76)90381-7
DO - 10.1016/0024-3205(76)90381-7
M3 - Article
VL - 19
SP - 117
EP - 123
JO - Life Sciences
JF - Life Sciences
SN - 0024-3205
IS - 1
ER -