HPLC separation and wavelength area ratios of more than 50 phenolic acids and flavonoids

W. L. Banwart, P. M. Porter, T. C. Granato, J. J. Hassett

Research output: Contribution to journalArticlepeer-review

53 Scopus citations


Relative retention times and wavelength area ratios for over 50 standard compounds were calculated using reverse-phase HPLC. The standard compounds analyzed included benzoic acids, cinnamic acids, benzene carboxylic acids, acetic acids, coumarins, benzaldehydes and a variety of flavonoid compounds including flavanones, flavones, isoflavones, and their glycosides. Each standard compound was chromatographed by three different gradient elutions. Compounds were detected by UV absorption at 254 nm and 280 nm. Relative retention times with respect to two different internal references and the 254nm: 280nm wavelength area ratio was determined for each standard. Soybean root and seed extracts were analyzed for the presence of the standard compounds using the chromatographic conditions described.

Original languageEnglish (US)
Pages (from-to)383-395
Number of pages13
JournalJournal of Chemical Ecology
Issue number3
StatePublished - Mar 1985


  • Glycine max
  • HPLC
  • area ratio
  • flavonoids
  • phenolic acids
  • retention time
  • soybean


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