How Reversed-Phase Liquid Chromatography Works

Mark R. Schure, Jake L. Rafferty, Ling Zhang, J. Ilja Siepmann

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


The keys to understanding reversed-phase liquid chromatography (LC) are provided at the molecular mechanism level as determined by high accuracy molecular simulation. The essential features of C 18 stationary-phase chains in contact with methanol-water and acetonitrile-water mixtures are discussed in the context of bonded-chain geometry, spatial distribution of alkane and alcohol solutes, retention mechanism, and retention thermodynamics. This tutorial is intended to be applicable to a wide audience ranging from occasional users of liquid chromatography to separation scientists.

Original languageEnglish (US)
Pages (from-to)630-637
Number of pages8
JournalLCGC North America
Issue number8
StatePublished - Aug 2013


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