Abstract
The keys to understanding reversed-phase liquid chromatography (LC) are provided at the molecular mechanism level as determined by high accuracy molecular simulation. The essential features of C 18 stationary-phase chains in contact with methanol-water and acetonitrile-water mixtures are discussed in the context of bonded-chain geometry, spatial distribution of alkane and alcohol solutes, retention mechanism, and retention thermodynamics. This tutorial is intended to be applicable to a wide audience ranging from occasional users of liquid chromatography to separation scientists.
Original language | English (US) |
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Pages (from-to) | 630-637 |
Number of pages | 8 |
Journal | LCGC North America |
Volume | 31 |
Issue number | 8 |
State | Published - Aug 2013 |