Host Characteristics and Bacterial Traits Predict Experimental Virulence for Escherichia coli Bloodstream Isolates from Patients with Urosepsis

James R. Johnson, Stephen Porter, Brian Johnston, Michael A. Kuskowski, Rachel R. Spurbeck, Harry L.T. Mobley, Deborah A. Williamson

Research output: Contribution to journalArticlepeer-review

77 Scopus citations

Abstract

Background. Extraintestinal Escherichia coli infections are common, costly, and potentially serious. A better understanding of their pathogenesis is needed. Methods. Sixty-seven E coli bloodstream isolates from adults with urosepsis (Seattle, WA; 1980s) underwent extensive molecular characterization and virulence assessment in 2 infection models (murine subcutaneous sepsis and moth larval lethality). Statistical comparisons were made among host characteristics, bacterial traits, and experimental virulence. Results. The 67 source patients were diverse for age, sex, and underlying medical and urological conditions. The corresponding E coli isolates exhibited diverse phylogenetic backgrounds and virulence profiles. Despite the E coli isolates′ common bloodstream origin, they exhibited a broad range of experimental virulence in mice and moth larvae, in patterns that (for the murine model only) corresponded significantly with host characteristics and bacterial traits. The most highly mouse-lethal strains were enriched with classic “urovirulence” traits and typically were from younger women with anatomically and functionally normal urinary tracts. The 2 animal models corresponded poorly with one another. Conclusions. Host compromise, including older age and urinary tract abnormalities, allows comparatively low-virulence E coli strains to cause urosepsis. Multiple E coli traits predict both experimental and epidemiological virulence. The larval lethality model cannot be a substitute for the murine sepsis model.

Original languageEnglish (US)
Article numberofv083
JournalOpen Forum Infectious Diseases
Volume2
Issue number3
DOIs
StatePublished - Jun 1 2015

Bibliographical note

Funding Information:
We thank Jack Erickson (Minneapolis VA Medical Center) who helped to create the images. Financial support. This material is based upon work supported by Office of Research and Development, Medical Research Service, Department of Veterans Affairs, grant numbers 1 I01 CX000192-01 and 1 I01 CX000920-01 (to J. R. J.). Potential conflicts of interest. All authors: No reported conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest.

Publisher Copyright:
© 2015 Oxford University Press. All rights reserved.

Keywords

  • Escherichia coli infections
  • Galleria mellonella
  • host compromise
  • insect model
  • mouse model
  • phylogenetic group urosepsis
  • sepsis
  • virulence
  • virulence factors
  • virulence genes

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