Hollow fiber bioartificial liver utilizing collagen-entrapped porcine hepatocyte spheroids

Florence J. Wu, Julie R. Friend, Arye Lazar, Henry J. Mann, Rory P Remmel, Frank B. Cerra, Wei-Shou Hu

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

A xenogeneic hollow fiber bioreactor utilizing collagen-entrapped dispersed hepatocytes has been developed as an extracorporeal bioartificial liver (BAL) for potential treatment of acute human fulminant hepatitis. Prolonged viability, enhanced liver-specific functions, and differentiated state have been observed in primary porcine hepatocytes cultivated as spheroids compared to dispersed hepatocytes plated on a monolayer. Entrapment of spheroids into the BAL can potentially improve performance over the existing device. Therefore, studies were conducted to evaluate the feasibility of utilizing spheroids as the functionally active component of the hybrid device. Confocal microscopy indicated high viability of spheroids entrapped into cylindrical collagen gel.

Original languageEnglish (US)
Pages (from-to)34-44
Number of pages11
JournalBiotechnology and bioengineering
Volume52
Issue number1
DOIs
StatePublished - Oct 5 1996

Keywords

  • albumin synthesis
  • bioartificial liver
  • bioreactor
  • collagen
  • glucuronidation
  • hepatocyte spheroid
  • hollow fiber
  • porcine hepatocyte
  • ureagenesis

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