Hollow fiber bioartificial liver utilizing collagen-entrapped porcine hepatocyte spheroids

Florence J. Wu, Julie R. Friend, Arye Lazar, Henry J. Mann, Rory P Remmel, Frank B. Cerra, Wei-Shou Hu

Research output: Contribution to journalArticlepeer-review

49 Scopus citations


A xenogeneic hollow fiber bioreactor utilizing collagen-entrapped dispersed hepatocytes has been developed as an extracorporeal bioartificial liver (BAL) for potential treatment of acute human fulminant hepatitis. Prolonged viability, enhanced liver-specific functions, and differentiated state have been observed in primary porcine hepatocytes cultivated as spheroids compared to dispersed hepatocytes plated on a monolayer. Entrapment of spheroids into the BAL can potentially improve performance over the existing device. Therefore, studies were conducted to evaluate the feasibility of utilizing spheroids as the functionally active component of the hybrid device. Confocal microscopy indicated high viability of spheroids entrapped into cylindrical collagen gel.

Original languageEnglish (US)
Pages (from-to)34-44
Number of pages11
JournalBiotechnology and bioengineering
Issue number1
StatePublished - Oct 5 1996


  • albumin synthesis
  • bioartificial liver
  • bioreactor
  • collagen
  • glucuronidation
  • hepatocyte spheroid
  • hollow fiber
  • porcine hepatocyte
  • ureagenesis


Dive into the research topics of 'Hollow fiber bioartificial liver utilizing collagen-entrapped porcine hepatocyte spheroids'. Together they form a unique fingerprint.

Cite this