HLA-G1+ Expression in GGTA1KO Pigs Suppresses Human and Monkey Anti-Pig T, B and NK Cell Responses

Joseph Sushil Rao, Nora Hosny, Ramesh Kumbha, Raza Ali Naqvi, Amar Singh, Zachary Swanson, Heather Levy, Anders W. Matson, Magie Steinhoff, Nicole Forneris, Eric Walters, Bernhard J. Hering, Christopher Burlak

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Abstract

The human leukocyte antigen G1 (HLA-G1), a non-classical class I major histocompatibility complex (MHC-I) protein, is a potent immunomodulatory molecule at the maternal/fetal interface and other environments to regulate the cellular immune response. We created GGTA1-/HLAG1+ pigs to explore their use as organ and cell donors that may extend xenograft survival and function in both preclinical nonhuman primate (NHP) models and future clinical trials. In the present study, HLA-G1 was expressed from the porcine ROSA26 locus by homology directed repair (HDR) mediated knock-in (KI) with simultaneous deletion of α-1-3-galactotransferase gene (GGTA1; GTKO) using the clustered regularly interspersed palindromic repeats (CRISPR)/CRISPR associated protein 9 (Cas9) (CRISPR/Cas9) gene-editing system. GTKO/HLAG1+ pigs showing immune inhibitory functions were generated through somatic cell nuclear transfer (SCNT). The presence of HLA-G1 at the ROSA26 locus and the deletion of GGTA1 were confirmed by next generation sequencing (NGS) and Sanger’s sequencing. Fibroblasts from piglets, biopsies from transplantable organs, and islets were positive for HLA-G1 expression by confocal microscopy, flow cytometry, or q-PCR. The expression of cell surface HLA-G1 molecule associated with endogenous β2-microglobulin (β2m) was confirmed by staining genetically engineered cells with fluorescently labeled recombinant ILT2 protein. Fibroblasts obtained from GTKO/HLAG1+ pigs were shown to modulate the immune response by lowering IFN-γ production by T cells and proliferation of CD4+ and CD8+ T cells, B cells and natural killer (NK) cells, as well as by augmenting phosphorylation of Src homology region 2 domain-containing phosphatase-2 (SHP-2), which plays a central role in immune suppression. Islets isolated from GTKO/HLA-G1+ genetically engineered pigs and transplanted into streptozotocin-diabetic nude mice restored normoglycemia, suggesting that the expression of HLA-G1 did not interfere with their ability to reverse diabetes. The findings presented here suggest that the HLA-G1+ transgene can be stably expressed from the ROSA26 locus of non-fetal maternal tissue at the cell surface. By providing an immunomodulatory signal, expression of HLA-G1+ may extend survival of porcine pancreatic islet and organ xenografts.

Original languageEnglish (US)
Article number730545
JournalFrontiers in immunology
Volume12
DOIs
StatePublished - Sep 9 2021

Bibliographical note

Funding Information:
This study was funded by sponsored research agreements governed by the University of Minnesota with Diabetes Free,

Funding Information:
We would like to acknowledge the contributions by Randall Prather Ph.D., Kevin Wells Ph.D., Kristin Whitworth Ph.D., Melissa Samuel and the staff at the National Swine Resource and Research Center for cloning and caring for the pigs used in this study and their funding from the National Institute of Health, NIAID and NHLBI (U42OD011140). We would also like to thank Melanie Graham MPH, PhD and the staff of the Preclinical Research Center (PCRC), University of Minnesota, St. Paul, Minnesota for their support in this project.

Publisher Copyright:
© Copyright © 2021 Rao, Hosny, Kumbha, Naqvi, Singh, Swanson, Levy, Matson, Steinhoff, Forneris, Walters, Hering and Burlak.

Keywords

  • CRIPSR/Cas9
  • HLAG-1
  • islet
  • porcine
  • xenotransplantation

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

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