Histone H2AX and the small RNA pathway modulate both non-homologous end-joining and homologous recombination in plants

Yiping Qi, Yong Zhang, Joshua A. Baller, Daniel F Voytas

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Using a zinc finger nuclease (ZFN) that creates a site-specific DNA double-strand break (DSB) at the endogenous ADH1 locus, we provide genetic evidence that histone H2AX and DSB-induced small RNAs modulate DNA repair by both non-homologous end-joining (NHEJ) and homologous recombination (HR) in the model plant Arabidopsis thaliana.

Original languageEnglish (US)
Pages (from-to)9-14
Number of pages6
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume783
DOIs
StatePublished - Jan 1 2016

Bibliographical note

Funding Information:
We thank all members of the Voytas lab for helpful discussions and suggestions. This work is supported by grants from the National Science Foundation ( MCB 0209818 and DBI 0923827 ) to DFV, the National Science Foundation of China ( 31271420 and 31330017 ) and the National Transgenic Major Project ( 2014ZX0801003B-002 ) to YZ, and startup funds provided by East Carolina University to YQ.

Publisher Copyright:
© 2015 Published by Elsevier B.V.

Keywords

  • Arabidopsis thaliana
  • DNA double-strand break (DSB) repair
  • H2AX
  • Small RNA
  • Zinc finger nuclease (ZFN)

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