Histone H2AX and the small RNA pathway modulate both non-homologous end-joining and homologous recombination in plants

Yiping Qi, Yong Zhang, Joshua A. Baller, Daniel F Voytas

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Using a zinc finger nuclease (ZFN) that creates a site-specific DNA double-strand break (DSB) at the endogenous ADH1 locus, we provide genetic evidence that histone H2AX and DSB-induced small RNAs modulate DNA repair by both non-homologous end-joining (NHEJ) and homologous recombination (HR) in the model plant Arabidopsis thaliana.

Original languageEnglish (US)
Pages (from-to)9-14
Number of pages6
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume783
DOIs
StatePublished - Jan 1 2016

Fingerprint

Genetic Loci
Double-Stranded DNA Breaks
Homologous Recombination
Zinc Fingers
Arabidopsis
DNA Repair
Histones
RNA

Keywords

  • Arabidopsis thaliana
  • DNA double-strand break (DSB) repair
  • H2AX
  • Small RNA
  • Zinc finger nuclease (ZFN)

Cite this

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AU - Qi, Yiping

AU - Zhang, Yong

AU - Baller, Joshua A.

AU - Voytas, Daniel F

PY - 2016/1/1

Y1 - 2016/1/1

N2 - Using a zinc finger nuclease (ZFN) that creates a site-specific DNA double-strand break (DSB) at the endogenous ADH1 locus, we provide genetic evidence that histone H2AX and DSB-induced small RNAs modulate DNA repair by both non-homologous end-joining (NHEJ) and homologous recombination (HR) in the model plant Arabidopsis thaliana.

AB - Using a zinc finger nuclease (ZFN) that creates a site-specific DNA double-strand break (DSB) at the endogenous ADH1 locus, we provide genetic evidence that histone H2AX and DSB-induced small RNAs modulate DNA repair by both non-homologous end-joining (NHEJ) and homologous recombination (HR) in the model plant Arabidopsis thaliana.

KW - Arabidopsis thaliana

KW - DNA double-strand break (DSB) repair

KW - H2AX

KW - Small RNA

KW - Zinc finger nuclease (ZFN)

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