Using a zinc finger nuclease (ZFN) that creates a site-specific DNA double-strand break (DSB) at the endogenous ADH1 locus, we provide genetic evidence that histone H2AX and DSB-induced small RNAs modulate DNA repair by both non-homologous end-joining (NHEJ) and homologous recombination (HR) in the model plant Arabidopsis thaliana.
|Original language||English (US)|
|Number of pages||6|
|Journal||Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis|
|State||Published - Jan 1 2016|
Bibliographical noteFunding Information:
We thank all members of the Voytas lab for helpful discussions and suggestions. This work is supported by grants from the National Science Foundation ( MCB 0209818 and DBI 0923827 ) to DFV, the National Science Foundation of China ( 31271420 and 31330017 ) and the National Transgenic Major Project ( 2014ZX0801003B-002 ) to YZ, and startup funds provided by East Carolina University to YQ.
© 2015 Published by Elsevier B.V.
- Arabidopsis thaliana
- DNA double-strand break (DSB) repair
- Small RNA
- Zinc finger nuclease (ZFN)